Spatially controlled cell engineering on biodegradable polymer surfaces
Spatially controlled cell engineering on biodegradable polymer surfaces
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DOI：
10.1096/fasebj.12.14.1447
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发表时间：
1998-11
期刊：
影响因子：
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通讯作者：
N. Patel;Robert Padera;G. Sanders;S. Cannizzaro;M. Davies;R. Langer;C. Roberts;S. Tendler;Philip M. Williams;K. Shakesheff
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文献类型：
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作者：
N. Patel;Robert Padera;G. Sanders;S. Cannizzaro;M. Davies;R. Langer;C. Roberts;S. Tendler;Philip M. Williams;K. Shakesheff
控制细胞和模板表面之间受体介导的相互作用是许多组织工程过程的核心原则 (1-3)。经工程设计以呈现细胞粘附配体的生物材料表面会经历整合素介导的与细胞的分子相互作用 (1,4,5)，刺激细胞扩散和分化 (6-8)。这提供了一种模仿自然细胞与基质相互作用的机制。通过制造配体空间分布仅限于微米级图案特征的表面，可以实现对细胞相互作用的进一步复杂控制（9-14）。图案化技术有望促进空间控制的组织工程，并应用于高度组织化的组织再生。这些新应用需要在生物相容性和可生物降解的模板上形成配体模式，在通过代谢去除之前控制组织再生过程。我们开发了一种在可生物降解的嵌段共聚物聚丙交酯-聚乙二醇表面上生成任何生物素化配体的微米级图案的方法。该技术实现了纳米级精度的生物分子沉积控制。当使用这些模板培养牛主动脉内皮细胞和 PC12 神经细胞时，观察到对细胞发育的空间控制。此外，可生物降解聚合物表面上的神经突延伸是由含有 IKVAV 序列 (15, 16) 的肽组成的模式特征引导的，这表明可以实现对可生物降解生物材料上神经再生的定向控制。-Patel, N.、Padera, R.、Sanders, G. H. W.、Cannizzaro, S. M.、Davies, M. C.、Langer, R.、Roberts, C. J., Tendler, S. J. B.、Williams, P. M. 和 Shakesheff, K. M. 可生物降解聚合物表面的空间控制细胞工程。 FASEB J. 12, 1447–1454 (1998)
Controlling receptor‐mediated interactions between cells and template surfaces is a central principle in many tissue engineering procedures (1–3). Biomaterial surfaces engineered to present cell adhesion ligands undergo integrin‐mediated molecular interactions with cells (1, 4, 5), stimulating cell spreading, and differentiation (6–8). This provides a mechanism for mimicking natural cell‐to‐matrix interactions. Further sophistication in the control of cell interactions can be achieved by fabricating surfaces on which the spatial distribution of ligands is restricted to micron‐scale pattern features (9–14). Patterning technology promises to facilitate spatially controlled tissue engineering with applications in the regeneration of highly organized tissues. These new applications require the formation of ligand patterns on biocompatible and biodegradable templates, which control tissue regeneration processes, before removal by metabolism. We have developed a method of generating micron‐scale patterns of any biotinylated ligand on the surface of a biodegradable block copolymer, polylactide‐poly(ethylene glycol). The technique achieves control of biomolecule deposition with nanometer precision. Spatial control over cell development has been observed when using these templates to culture bovine aortic endothelial cells and PC12 nerve cells. Furthermore, neurite extension on the biodegradable polymer surface is directed by pattern features composed of peptides containing the IKVAV sequence (15, 16), suggesting that directional control over nerve regeneration on biodegradable biomaterials can be achieved.—Patel, N., Padera, R., Sanders, G. H. W., Cannizzaro, S. M., Davies, M. C., Langer, R., Roberts, C. J., Tendler, S. J. B., Williams, P. M., and Shakesheff, K. M. Spatially controlled cell engineering on biodegradable polymer surfaces. FASEB J. 12, 1447–1454 (1998)