Detection and cultivation of intestinal trichomonads of squirrel monkeys (Saimiri sciureus)
Detection and cultivation of intestinal trichomonads of squirrel monkeys (Saimiri sciureus)
复制标题
松鼠猴（Saimiri sciureus）肠道滴虫的检测与培养
DOI：
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发表时间：
1985
影响因子：
2.4
通讯作者：
W. A. Gardner
中科院分区：
文献类型：
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作者：
F. Pindak;M. D. Pindak;C. Abee;W. A. Gardner
对松鼠猴粪便样本的常规检查表明，肠道毛滴虫可能是这些动物的常见居民。为了进行这些观察，粪便悬浮液和培养物的显微镜检查表明，从10只一组的300多只动物中任意选择的30只动物中滴虫的发生率为100%。最突出的物种是人五毛滴虫。一个尚未完全特征的三毛滴虫也被发现在几个场合。在培养物中建立单个菌株的主要障碍是样品中存在细菌和真菌植物群。然而，通过将粪便悬浮液接种到含有适当配制的培养基和高浓度抗生素的组织培养物中，从28只动物中获得了丰富的培养物。在几个无人值守的文化保持在室温下，鞭毛虫保留运动至少4个月。这种长期生存可能解释了寄生虫在一个封闭的动物群落中广泛发生的原因。
Routine examinations of fecal samples from squirrel monkeys suggested that intestinal trichomonads might be common inhabitants of these animals. In pursuit of these observations, microscopic examination of fecal suspensions and cultures have demonstrated a 100% incidence of trichomonads in 30 arbitrarily selected animals from a colony of more than 300 housed in groups of ten. The most prominent species was Pentatrichomonas hominis. A not yet fully characterized tritrichomonad was also found on several occasions. The main obstacle in establishing individual strains in culture was the presence of bacterial and fungal flora in the samples. Nevertheless, abundant cultures were obtained from 28 animals by inoculation of fecal suspensions into tissue cultures with appropriately formulated medium and high concentration of antibiotics. In several unattended cultures maintained at room temperature, the flagellates retained motility for at least 4 months. This long survival may explain the widespread occurrence of the parasites within a confined animal community.