Stimulation of growth of human melanocytes by tumor promoters.
Stimulation of growth of human melanocytes by tumor promoters.
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肿瘤促进剂刺激人类黑色素细胞的生长。
DOI：
10.1093/carcin/4.6.779
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发表时间：
1983
期刊：
影响因子：
4.7
通讯作者：
Weinstein,IB
中科院分区：
文献类型：
--
作者：
Eisinger,M;Marko,O;Weinstein,IB
在以前的研究中，我们发现，当加入到原代正常人表皮培养物中时，12-O-十四酰基佛波醇13-乙酸酯（IPA）（10 ng/ml）选择性地抑制否则占优势的角质形成细胞群体的生长，并且这与正常黑素细胞的生长有关。目前的研究表明，如果培养基中含有TPA，这些黑素细胞随后可以生长至少30代，但如果除去该化合物，细胞停止分裂。一系列佛波醇酯支持正常人黑素细胞生长的能力通常与它们在小鼠皮肤上的肿瘤促进活性相关。两种结构上不相关的化合物最近被证明对小鼠皮肤具有肿瘤促进活性，即杀鱼素和阿朴氏毒素，也支持黑素细胞生长。另一方面，一些多肽生长因子不能替代TPA。由于人黑色素瘤细胞系在没有肿瘤促进剂的情况下生长旺盛，我们的研究结果表明，黑色素细胞的恶性转化与某些未鉴定的内源性生长因子的自主性有关。
In previous studies we found that, when added to primary normal human epidermal cultures, 12-O-tetradecanoyl phor-bol 13-acetate (IPA) (10 ng/ml) selectively suppresses the growth of the otherwise predominant keratinocyte cell population and that this is associated with the outgrowth of normal melanocytes. The present study indicates that these melanocytes can be subsequently grown for at least 30 passages if the medium contains TPA, but if the compound is removed the cells cease to divide. The ability of a series of phorbol esters to support the growth of normal human melanocytes correlates, in general, with their tumor promoting activity on mouse skin. Two structurally unrelated types of compounds which have recently been shown to have tumor promoting activity on mouse skin, teleocidin and aplysiatoxin, also support melanocyte growth. On the other hand, several polypeptide growth factors could not substitute for TPA. Since human melanoma cell lines grow vigorously in the absence of tumor promoters our results suggest that the malignant transformation of melanocytes is associated with the acquisition of autonomy from certain unidentified endogenus growth factors.