Pathogenesis of Bohle Iridovirus (Genus Ranavirus) in Experimentally Infected Juvenile Eastern Water Dragons (Intellagama lesueurii lesueurii)
Pathogenesis of Bohle Iridovirus (Genus Ranavirus) in Experimentally Infected Juvenile Eastern Water Dragons (Intellagama lesueurii lesueurii)
复制标题
实验感染的幼年东部水龙 (Intellagama lesueurii lesueurii) 中博勒虹彩病毒（蛙病毒属）的发病机制
DOI：
10.1177/0300985818823666
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发表时间：
2019
影响因子：
2.4
通讯作者：
E. Ariel
中科院分区：
文献类型：
--
作者：
A. Maclaine;M. Forzán;N. Mashkour;Jennifer L. Scott;E. Ariel
东部水龙幼龙（Intellagama lesueurii lesueurii）极易感染Bohle iridovirus (BIV)，这是一种首次从澳大利亚汤斯维尔的华美穴居蛙中分离到的ranavirus。为了研究东部水龙感染BIV的进展情况，采用104.33 TCID50剂量对11只圈养水龙进行口腔接种，分别于感染后3、6、8、10、12和14天实施安乐死。在3 dpi时通过聚合酶链反应（PCR）在肝脏、肾脏和肛肠拭子中检测病毒DNA。3 dpi时，舌黏膜下层及肝黏膜可见轻度淋巴细胞浸润。免疫组织化学（IHC）首先在脾坏死灶和胞浆内包涵的肝细胞或罕见的6 dpi单细胞坏死中发现病毒抗原。到14 dpi时，发现IHC阳性标记与多个组织的病变有关。从14 dpi安乐死的个体中选择组织，使用原位杂交（ISH）进行探针。ISH标记与免疫组化检测的位置和模式相匹配。根据病变严重程度和病毒检测，东部水龙的BIV感染的进展似乎始于脾脏，其次是肝脏，然后是其他器官，如肾脏、胰腺、口腔黏膜和皮肤。在肛肠拭子和肝脏和肾脏组织样本中早期检测到ranaviral DNA表明，在出现临床症状之前的疾病早期阶段以及整个感染过程中，这些是可靠的诊断样本来源。
Juvenile eastern water dragons (Intellagama lesueurii lesueurii) are highly susceptible to infection with Bohle iridovirus (BIV), a species of ranavirus first isolated from ornate burrowing frogs in Townsville, Australia. To investigate the progression of BIV infection in eastern water dragons, 11 captive-bred juveniles were orally inoculated with a dose of 104.33 TCID50 and euthanized at 3, 6, 8, 10, 12, and 14 days postinfection (dpi). Viral DNA was detected via polymerase chain reaction (PCR) in the liver, kidney, and cloacal swabs at 3 dpi. Mild lymphocytic infiltration was observed in the submucosa and mucosa of the tongue and liver at 3 dpi. Immunohistochemistry (IHC) first identified viral antigen in foci of splenic necrosis and in hepatocytes with intracytoplasmic inclusion or rare single-cell necrosis at 6 dpi. By 14 dpi, positive IHC labeling was found in association with lesions in multiple tissues. Selected tissues from an individual euthanized at 14 dpi were probed using in situ hybridization (ISH). The ISH labeling matched the location and pattern detected by IHC. The progression of BIV infection in eastern water dragons, based on lesion severity and virus detection, appears to start in the spleen, followed by the liver, then other organs such as the kidney, pancreas, oral mucosa, and skin. The early detection of ranaviral DNA in cloacal swabs and liver and kidney tissue samples suggests these to be a reliable source of diagnostic samples in the early stage of disease before the appearance of clinical signs, as well as throughout the infection.