IDENTIFICATION OF 2 TYPES OF TUMOR-NECROSIS-FACTOR RECEPTORS ON HUMAN CELL-LINES BY MONOCLONAL-ANTIBODIES
IDENTIFICATION OF 2 TYPES OF TUMOR-NECROSIS-FACTOR RECEPTORS ON HUMAN CELL-LINES BY MONOCLONAL-ANTIBODIES
复制标题
DOI：
10.1073/pnas.87.8.3127
复制
发表时间：
1990-04-01
影响因子：
11.1
通讯作者：
LOETSCHER, H
中科院分区：
文献类型：
--
作者：
BROCKHAUS, M;SCHOENFELD, HJ;LOETSCHER, H
多效性细胞因子/淋巴因子肿瘤坏死因子（TNF）通过与特异性细胞表面受体结合发挥其功能。我们制备了两组抗HL-60（htr-mAb系列）和U-937（utr-mAb系列）细胞系TNF结合蛋白的单克隆抗体（mAb）。htr抗体抑制125 I标记的TNF-α的结合。对HL-60细胞的作用，而它们阻断TNF-α。与几种腺癌细胞系（HEp-2、HeLa和MCF 7）几乎完全结合。相反，utr抗体对TNF-α没有影响。结合腺癌细胞系，但部分抑制TNF-α。结合HL-60和U-937细胞。然而，htr-9和utr-1抗体组合完全抑制TNF-α。结合HL-60和U-937细胞。TNF-β的结合对HEp-2和U-937细胞的作用也被htr和utr抗体抑制。htr和utr mAb对TNF敏感的鼠细胞系L929和WEHI 164均无影响。流式细胞术研究表明，单克隆抗体htr-9和utr-1检测两个不同的TNF-结合位点的人细胞系。免疫印迹和免疫沉淀分析表明，mAbs htr-9和utr-1分别识别约55 kDa和75 kDa的蛋白质。这些数据为存在两种不同的TNF受体分子提供了证据，这两种不同的TNF受体分子在不同程度上有助于不同人细胞的TNF结合。
The pleiotropic cyto/lymphokine tumor necrosis factor (TNF) exerts its functions by binding to specific cell-surface receptors. We have prepared two sets of monoclonal antibodies (mAbs) against TNF-binding proteins from the HL-60 (htr-mAb series) and U-937 (utr-mAb series) cell lines. The htr antibodies inhibit the binding of 125I-labeled TNF-.alpha. to HL-60 cells only partially, whereas they block the TNF-.alpha. binding to several adenocarcinoma cell lines (HEp-2, HeLa, and MCF7) almost completely. In contrast, the utr antibodies have no effect on TNF-.alpha. binding to the adenocarcinoma cell lines but partially inhibit TNF-.alpha. binding to HL-60 and U-937 cells. However, htr-9 and utr-1 antibodies in combination fully inhibit the TNF-.alpha. binding to HL-60 and U-937 cells. The binding of TNF-.beta. to HEp-2 and U-937 cells is also inhibited by htr and utr antibodies. Neither htr nor utr mAb has an effect on the TNF-sensitive murine cell lines L929 and WEHI 164. Flow cytometry studies show that mAbs htr-9 and utr-1 detect two distinct TNF-binding sites on human cell lines. Immunologic blot and immunoprecipitation analyses indicate that mAbs htr-9 and utr-1 recognize proteins of .apprxeq.55 kDa and 75 kDa, respectively. These data provide evidence for the existence of two distinct TNF receptor molecules that contribute to varying extent to the TNF binding by different human cells.