Replication protein RPA2A regulates floral transition by cooperating with PRC2 in Arabidopsis
Replication protein RPA2A regulates floral transition by cooperating with PRC2 in Arabidopsis
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拟南芥中复制蛋白 RPA2A 通过与 PRC2 合作调节花转变
DOI：
10.1111/nph.18279
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Hongchun Yang
中科院分区：
文献类型：
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作者：
Xiaoling Zhang;Wenjuan Li;Yue Liu;Yanzhuo Li;Yang Li;W;ong Yang;Xiangsong Chen;Limin Pi;Hongchun Yang
RPA 2A是拟南芥中保守的异源三聚体复制蛋白A（RPA）的一个亚基，RPA是DNA复制过程中与单链DNA结合的重要复制体组分。RPA 2A控制一系列发育过程，但其潜在机制在很大程度上尚不清楚。在这里，我们表明RPA 2A抑制关键开花基因，包括FLOWERING LOCUS T（FT）、AGAMOUS（AG）和AGAMOUS LIKE 71（AGL 71），通过与PRC 2复合体合作来抑制花转变。RPA 2A在分裂细胞中强烈表达，并且是正确DNA复制所必需的。RPA 2A基因突变导致植物早花，这在分子和遗传上依赖于包括FT在内的关键成花基因的异位表达。RPA 2A和PRC 2具有共同的靶基因，包括FT、AG和AGL 71，使用遗传分析、转录组分析和H3 K27 me 3 ChIP-seq分析支持。此外，RPA 2A与PRC 2组分CLF、EMF 2和MSI 1物理相互作用，其将CLF募集到FT、AG和AGL 71的染色质基因座。总之，我们的研究结果表明，复制蛋白RPA 2A招募PRC 2关键开花基因通过物理蛋白质相互作用，从而抑制这些基因的表达，抑制花的转变在拟南芥。
RPA2A is a subunit of the conserved heterotrimeric replication protein A (RPA) in Arabidopsis, which is an essential replisome component that binds to single-stranded DNA during DNA replication. RPA2A controls a set of developmental processes, but the underlying mechanism is largely unknown. Here we show that RPA2A represses key flowering genes including FLOWERING LOCUS T (FT), AGAMOUS (AG) and AGAMOUS LIKE 71 (AGL71) to suppress floral transition by cooperating with the PRC2 complex. RPA2A is vigorously expressed in dividing cells and required for correct DNA replication. Mutation of RPA2A leads to early flowering, which is dependent on ectopic expression of key flowering genes including FT molecularly and genetically. RPA2A and PRC2 have common target genes including FT, AG and AGL71 supported using genetic analysis, transcriptome profiling and H3K27me3 ChIP-seq analysis. Furthermore, RPA2A physically interacts with PRC2 components CLF, EMF2 and MSI1, which recruits CLF to the chromatin loci of FT, AG and AGL71. Together, our results show that the replication protein RPA2A recruits PRC2 to key flowering genes through physical protein interaction, thereby repressing the expression of these genes to suppress floral transition in Arabidopsis.