INTRACYTOPLASMIC SPERM INJECTION IN THE MOUSE
INTRACYTOPLASMIC SPERM INJECTION IN THE MOUSE
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DOI：
10.1095/biolreprod52.4.709
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发表时间：
1995-04-01
影响因子：
3.6
通讯作者：
YANAGIMACHI, R
中科院分区：
文献类型：
--
作者：
KIMURA, Y;YANAGIMACHI, R
当使用压电驱动的微量移液器代替机械驱动的传统移液器时，小鼠胞浆内精子注射（ICSI）获得成功。80%的精子注射卵母细胞存活，其中约70%在体外发育成囊胚。当106个2- 4-细胞阶段的胚胎被转移到8个自然交配的寄养母亲身上时，30%的胚胎（25- 43%，取决于宿主）达到了足月。除了两个出生后不久就被吃掉，所有的年轻人（30只幼崽）都长成了正常的成年人。对小鼠进行这种类型的研究可能会增加对受精过程和ICSI工作原理的理解。
Intracytoplasmic sperm injection (ICSI) was successful in the mouse when a piezo-driven micropipette was used instead of a mechanically driven conventional pipette. Eighty percent of sperm-injected oocytes survived, and approximately 70% of them developed into blastocysts in vitro. When 106 embryos at the 2- to 4-cell stage were transferred to eight naturally mated foster mothers, 30% of the embryos (25-43%, depending on the host) reached the full term. Except for two that were cannibalized soon after birth, all of the young (30 pups) grew into normal adults. Studies of this type on the mouse may increase understanding of the fertilization process and of how ICSI works.