Invasive cytotrophoblasts manifest evidence of oxidative stress in preeclampsia
Invasive cytotrophoblasts manifest evidence of oxidative stress in preeclampsia
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DOI：
10.1016/s0002-9440(10)64733-5
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发表时间：
2000-01-01
影响因子：
6
通讯作者：
Zhou, Y
中科院分区：
文献类型：
--
作者：
Many, A;Hubel, CA;Zhou, Y
在子痫前期，胎盘灌注不良可能导致母体内皮功能障碍，但涉及的途径在很大程度上是未知的。候选的胎盘介质包括释放到母体循环中的氧化应激产物。黄嘌呤氧化酶通过产生超氧化物自由基（superoxide; O-2）参与了缺血再灌注损伤。我们检查了子痫前期孕妇的胎盘和胎盘床切面和/或活检，以验证黄嘌呤氧化酶是子痫前期孕妇胎盘氧化应激介质的假设。黄嘌呤脱氢酶/黄嘌呤氧化酶全酶的表达和黄嘌呤氧化酶（已知产生活性氧的同种异构体）的活性在子痫前期妇女的细胞滋养细胞亚群中增加。此外，清除黄嘌呤氧化酶产生的超氧化物的超氧化物歧化酶在同一细胞中的表达减少。此外，在这些细胞和绒毛血管中，硝基酪氨酸的荧光免疫染色增加，这表明超氧化物-一氧化氮相互作用在体内形成过氧亚硝酸盐阴离子（ONOO-）。因此，我们的数据表明胎盘细胞在子痫前期产生活性氧的能力增加。
In preeclampsia, poor placental perfusion may result in maternal endothelial dysfunction, but the pathways involved ed are largely unknown. Candidate placental mediators include products of oxidative stress released into the maternal circulation. Xanthine oxidase has been implicated in postischemic-reperfusion injury via the generation of superoxide action radicals (superoxide; O-2(.-)) and hydrogen peroxide, We examined placentas and placental bed curettings and/or biopsies from preeclamptic control pregnant women to test the hypothesis that xanthine oxidase is a mediator of oxidative stress in placentas from women with preeclampsia. The expression of xanthine dehydrogenase/xanthine oxidase holoenzyme and the activity of xanthine oxidase, the isoform known to generate reactive oxygen species, were increased in a subpopulation of cytotrophoblasts of preeclamptic women. Additionally, the expression of superoxide dismutase, which would scavenge superoxide produced by xanthine oxidase, was reduced in the same cells. Furthermore, fluorescence immunostaining for nitrotyrosine, which was suggestive of superoxide-nitric oxide interactions to form peroxynitrite anion (ONOO-) in vivo, was increased in these cells and in villous vessels. Thus, our data indicate an increased capacity of placental cells to generate reactive oxygen-species in preeclampsia.