Chromosome arrangement and nuclear architecture but not centromeric sequences are conserved between Arabidopsis thaliana and Arabidopsis lyrata
Chromosome arrangement and nuclear architecture but not centromeric sequences are conserved between Arabidopsis thaliana and Arabidopsis lyrata
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DOI：
10.1111/j.1365-313x.2006.02912.x
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发表时间：
2006-12-01
期刊：
影响因子：
7.2
通讯作者：
Schubert, Ingo
中科院分区：
文献类型：
--
作者：
Berr, Alexandre;Pecinka, Ales;Schubert, Ingo
与哺乳动物和果蝇所描述的情况相反，染色体领土（CT）的安排和体细胞同源配对在间期细胞核的拟南芥（n = 5）主要是随机的，除了一个更频繁的协会的染色体携带同源核仁组织者区域。为了找出这种染色体排列是否也是拟南芥属其他物种的特征，我们调查了拟南芥lyrata ssp。lyrata（n = 8），是A. thaliana.首先，我们测定了A.抒情琴。然后，我们证明了异染色质区和常染色质区的CT排列、同源配对和姐妹染色单体排列。lyrata间期核与A.拟南芥核因此，间期染色体的排列似乎是保守的两个类群之间的分歧约500万年前。由于A.虽然lyrata的核型与推测的祖先核型相似，但也可以预期其他近缘的菊科二倍体物种的间期染色体也有类似的排列。
In contrast to the situation described for mammals and Drosophila, chromosome territory (CT) arrangement and somatic homologous pairing in interphase nuclei of Arabidopsis thaliana (n = 5) are predominantly random except for a more frequent association of the chromosomes bearing a homologous nucleolus organizer region. To find out whether this chromosome arrangement is also characteristic for other species of the genus Arabidopsis, we investigated Arabidopsis lyrata ssp. lyrata (n = 8), one of the closest relatives of A. thaliana. First, we determined the size of each chromosome and chromosome arm, the sequence type of centromeric repeats and their distribution between individual centromeres and the position of the 5S/45S rDNA arrays in A. lyrata. Then we demonstrated that CT arrangement, homologous pairing and sister chromatid alignment of distinct euchromatic and/or heterochromatic regions within A. lyrata interphase nuclei are similar to that in A. thaliana nuclei. Thus, the arrangement of interphase chromosomes appears to be conserved between both taxa that diverged about 5 million years ago. Since the chromosomes of A. lyrata resemble those of the presumed ancestral karyotype, a similar arrangement of interphase chromosomes is also to be expected for other closely related diploid species of the Brassicaceae family.