Complex Gene Regulation Underlying Mineral Nutrient Homeostasis in Soybean Root Response to Acidity Stress
Complex Gene Regulation Underlying Mineral Nutrient Homeostasis in Soybean Root Response to Acidity Stress
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大豆根系对酸度胁迫反应中矿物质养分稳态的复杂基因调控
DOI：
10.3390/genes10050402
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发表时间：
2019-05-01
期刊：
影响因子：
3.5
通讯作者：
Liang, Cuiyue
中科院分区：
文献类型：
--
作者：
Chen, Qianqian;Wu, Weiwei;Liang, Cuiyue
质子毒性是限制作物生产的主要环境压力之一，并且由于人类活动而变得日益严重。为了了解耐酸机制，研究了大豆 (Glycine max) 响应长期酸胁迫的植物生长、矿质营养素积累和整体转录组变化。结果表明，酸性胁迫显着抑制大豆根系生长，但对地上部生长影响较小。此外，必需矿质营养素的浓度受到酸度胁迫的显着影响，主要在大豆器官和矿质营养素类型之间存在差异。酸性胁迫分别显着降低了叶片和根中磷（P）和钼（Mo）的浓度，根中氮（N）和钾（K）的浓度以及叶片中镁（Mg）的浓度。而叶和根中钙 (Ca)、硫酸盐 (S) 和铁 (Fe) 的浓度均增加。大豆根部的转录组分析鉴定出 419 个在酸性条件下上调和 555 个下调的基因。共有 38 个差异表达基因 (DEG) 参与矿质营养素运输。其中，所有检测到的5个GmPT、4个GmZIP、2个GmAMT和GmKUP以及GmIRT1、GmNramp5、GmVIT2.1、GmSKOR、GmTPK5和GmHKT1均在酸性胁迫下显着下调。此外，编码转录因子（例如 GmSTOP2s）并与 pH 统计代谢途径相关的基因的转录因酸度胁迫而显着上调。综上所述，它强烈表明维持 pH 值稳定和矿物质营养稳态是大豆应对酸性胁迫的适应性策略，这可能受到复杂的信号网络的调节。
Proton toxicity is one of the major environmental stresses limiting crop production and becomes increasingly serious because of anthropogenic activities. To understand acid tolerance mechanisms, the plant growth, mineral nutrients accumulation, and global transcriptome changes in soybean (Glycine max) in response to long-term acidity stress were investigated. Results showed that acidity stress significantly inhibited soybean root growth but exhibited slight effects on the shoot growth. Moreover, concentrations of essential mineral nutrients were significantly affected by acidity stress, mainly differing among soybean organs and mineral nutrient types. Concentrations of phosphorus (P) and molybdenum (Mo) in both leaves and roots, nitrogen (N), and potassium (K) in roots and magnesium (Mg) in leaves were significantly decreased by acidity stress, respectively. Whereas, concentrations of calcium (Ca), sulfate (S), and iron (Fe) were increased in both leaves and roots. Transcriptome analyses in soybean roots resulted in identification of 419 up-regulated and 555 down-regulated genes under acid conditions. A total of 38 differentially expressed genes (DEGs) were involved in mineral nutrients transportation. Among them, all the detected five GmPTs, four GmZIPs, two GmAMTs, and GmKUPs, together with GmIRT1, GmNramp5, GmVIT2.1, GmSKOR, GmTPK5, and GmHKT1, were significantly down-regulated by acidity stress. Moreover, the transcription of genes encoding transcription factors (e.g., GmSTOP2s) and associated with pH stat metabolic pathways was significantly up-regulated by acidity stress. Taken together, it strongly suggests that maintaining pH stat and mineral nutrient homeostasis are adaptive strategies of soybean responses to acidity stress, which might be regulated by a complex signaling network.