Following cellulose depolymerization in paper: comparison of size exclusion chromatography techniques
Following cellulose depolymerization in paper: comparison of size exclusion chromatography techniques
复制标题
DOI：
10.1007/s10570-011-9562-7
复制
发表时间：
2011-10-01
期刊：
影响因子：
5.7
通讯作者：
Lojewska, Joanna
中科院分区：
文献类型：
--
作者：
Lojewski, Tomasz;Zieba, Katarzyna;Lojewska, Joanna
本研究的重点是比较的结果，通过尺寸排阻色谱（SEC）检测系统，使用多角度激光散射（MALLS）和/或紫外-可见光（UV/维斯）检测器在纸基文物纤维素的分析。用于评价纸降解阶段和动力学的常用参数是通过SEC测量的重量（M（w））平均摩尔质量。这里要解决的主要问题是，通过各种技术和校准策略以及在各个实验室中获得的参数是否可以被普遍对待，以及差异的来源是什么。SEC-UV/维斯技术证明了实验室间相关性所证明的重现性结果。使用各种数据处理方法和SEC技术，它也表明，摩尔质量只应相对处理。基于从各种技术获得的M（w）值和用各种校准程序处理的动力学曲线之间的差异质疑使用SEC结果推导的动力学方程描述纸张降解的可靠性。
The study focuses on the comparison of the results obtained by size exclusion chromatography (SEC) detection systems using multiangle laser light scattering (MALLS) and/or ultraviolet-visible (UV/VIS) detectors in analyses of cellulose in paper-based heritage objects. The commonly applied parameter for the evaluation of paper degradation stage and kinetics is weight (M (w) ) average molar mass measured by SEC. The main problems addressed here are whether the parameters obtained by various techniques and calibration strategies and in various laboratories can be treated universally and what the sources of the discrepancies can be. The SEC-UV/VIS technique proved to give reproducible results as demonstrated by the interlaboratory correlations. Using various data processing methods and SEC techniques it was also shown that molar masses should only be treated relatively. The differences between the kinetic curves based on the M (w) values obtained from various techniques and processed with various calibration procedures question the reliability of kinetic equations derived using the SEC results to describe the paper degradation.