Identification and expression analysis of rice histone genes
Identification and expression analysis of rice histone genes
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水稻组蛋白基因的鉴定及表达分析
DOI：
10.1016/j.plaphy.2014.11.012
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发表时间：
2015-01-01
影响因子：
6.5
通讯作者：
Lai, Yan
中科院分区：
文献类型：
--
作者：
Hu, Yongfeng;Lai, Yan
组蛋白作为核小体的核心，是染色质的主要蛋白质成分。它们通过多个位点的共价修饰和组蛋白变体替换在基因调控中发挥重要作用。存在五个主要组蛋白家族：H1、H2A、H2B、H3 和 H4。每个家族内的蛋白质序列似乎高度保守。在本文中，我们鉴定了水稻（Oryza sativa）中的 60 个组蛋白，包括 14 个 H2A、15 个 H2B、16 个 H3、11 个 H4 和 4 个 H1。序列分析表明植物中的组蛋白序列比动物中的变化更大。有趣的是，我们发现了一种水稻特有的 H4 变体，它显示出多个氨基酸被经典蛋白质取代，并且在不同组织中低水平表达。表达分析表明，组蛋白基因的一个子集以相似的模式表达，并且其中许多对应激条件做出反应。具体来说，我们发现两个 H2A.Z 基因在叶子中受到胁迫下调，但在根部却没有，这表明它们可能参与胁迫反应。 (C) 2014 年 Elsevier Masson SAS。版权所有。
Histones, acting as the core of nucleosome, are the chief protein component of chromatin. They play an important role in gene regulation by covalent modification at several sites and histone variants replacement. Five major families of histones exist: H1, H2A, H2B, H3 and H4. The protein sequences within each family appear to be highly conserved. In this paper, we identified 60 histone proteins in rice (Oryza sativa) including 14 H2A, 15 H2B, 16 H3,11 H4 and 4 H1. Sequence analysis indicates that histone protein sequences in plant are more variable than in animal. Interestingly, we found a rice-specific H4 variant which showed several amino acid substitutions with canonical protein and was expressed in different tissues in a low level. Expression analysis indicates that a subset of histone genes were expressed in a similar pattern and many of them responded to stress conditions. Specifically, we found that two H2A.Z genes were down-regulated by stress in leaves but not in roots suggesting that they might be involved in stress response. (C) 2014 Elsevier Masson SAS. All rights reserved.