Use of at-line and in-situ near-infrared spectroscopy to monitor biomass in an industrial fed-batch Escherichia coli process
Use of at-line and in-situ near-infrared spectroscopy to monitor biomass in an industrial fed-batch Escherichia coli process
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DOI：
10.1002/bit.10383
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发表时间：
2002-11-20
影响因子：
3.8
通讯作者：
McNeil, B
中科院分区：
文献类型：
--
作者：
Arnold, SA;Gaensakoo, R;McNeil, B
生物过程监测的关键目标之一是实时了解生物反应器内的状况，即原位状况。近红外光谱 (NIRS) 能够在几乎不需要样品的情况下快速进行多分析物定量，因此可能适用于这一角色。在本研究中，研究了 NIRS 在复杂的分批补料工业大肠杆菌 (RV308/PHKY531) 过程中的应用。该过程经历一系列温度变化并剧烈搅拌和充气。这些条件可能会给原位 NIRS 带来额外的挑战。以关键分析物（生物量）的测量为例，从理论和实际应用的角度详细考虑近红外光谱仪的在线和原位使用之间的关系。这项研究表明，近红外光谱可以在线和原位使用，以获得良好的生物量预测模型。原位操作带来了特殊的挑战（波长区域的损失和噪声），这意味着需要进行信号优化研究。这表明，虽然在线建模过程可以为原位过程提供一些有用的信息，但存在明显的差异。这项研究表明，在极具挑战性的基质（类似于当前工业实践中遇到的基质）中使用 NIRS 是可能的，从而扩展了该领域之前的工作。 (C) 2002 年 Wiley 期刊公司
One of the key goals in bioprocess monitoring is to achieve real-time knowledge of conditions within the bioreactor, i.e., in-situ. Near-infrared spectroscopy (NIRS), with its ability to carry out multi-analyte quantification rapidly with little sample presentation, is potentially applicable in this role. In the present study, the application of NIRS to a complex, fed-batch industrial E. coli (RV308/PHKY531) process was investigated. This process undergoes a series of temperature changes and is vigorously agitated and aerated. These conditions can pose added challenges to in-situ NIRS. Using the measurement of a key analyte (biomass) as an illustration, the details of the relationship between the at-line and in-situ use of NIRS are considered from the viewpoint of both theory and practical application. This study shows that NIRS can be used both at-line and in-situ in order to achieve good predictive models for biomass. There are particular challenges imposed by in-situ operation (loss of wavelength regions and noise) which meant the need for signal optimisation studies. This showed that whilst the at-line modelling process may provide some useful information for the in-situ process, there were distinct differences. This study shows that the in-situ use of NIRS in a highly challenging matrix (similar to those encountered in current industrial practice) is possible, and thus extends previous works in the area. (C) 2002 Wiley Periodicals, Inc.