Fetal liver as a source of autologous progenitor cells for perinatal tissue engineering.
Fetal liver as a source of autologous progenitor cells for perinatal tissue engineering.
复制标题
胎儿肝脏作为围产期组织工程自体祖细胞的来源。
DOI：
10.1089/1076327041348419
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Flake,AlanW
中科院分区：
文献类型：
--
作者：
Krupnick,AlexanderS;Balsara,KekiR;Kreisel,Daniel;Riha,Markus;Gelman,AndrewE;Estives,MiguelS;Amin,KunjlataM;Rosengard,BruceR;Flake,AlanW
Mesenchymal progenitor cells, isolated from adult bone marrow, have been shown to have utility for autologous tissue engineering. The possibility of isolating from the fetal hematopoietic system a cell population with similar potential, which could be used for autologous reconstruction of prenatally diagnosed congenital anomalies, has not been explored to date. Liver stromal cells isolated from a portion of the right lateral hepatic lobe of midgestation fetal lambs were expandedin vitro. Passage 1 cells displayed a uniform fibroblast-like morphology but could be induced to differentiate into skeletal muscle, adipocytes, chondrocytes, and endothelial cells by selective medium supplementation. By manipulating the extracellular matrixin vitro, spontaneously contracting cardiac myocyte-like cells could be generated as well. Multilineage differentiation was confirmed by morphology, protein expression, and upregulation of lineage-specific mRNA. The potential for engineering myocardial tissue was then investigated by transplanting early-passage progenitor cells, organized on a three-dimensional matrix, into the ventricle of an immunocompromised rat utilizing a previously described model of left ventricular tissue engineering. Survival, incorporation into the host myocardium, and cardiomyocytic differentiation of the transplanted cells were confirmed. We have demonstrated that mesenchymal progenitor cells with multilineage potential can be isolated from the fetal liver and have potential utility for autologous tissue engineering.
