Orexin receptor-1 (OX-R1) immunoreactivity in chemically identified neurons of the hypothalamus:: focus on orexin targets involved in control of food and water intake
Orexin receptor-1 (OX-R1) immunoreactivity in chemically identified neurons of the hypothalamus:: focus on orexin targets involved in control of food and water intake
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DOI：
10.1046/j.0953-816x.2001.01859.x
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发表时间：
2002-01-01
影响因子：
3.4
通讯作者：
Meister, B
中科院分区：
文献类型：
--
作者：
Bäckberg, M;Hervieu, G;Meister, B
食欲素- a和食欲素- b神经肽产生于下丘脑外侧区神经元，参与调节食物/水的摄入和睡眠-觉醒控制。食欲素作用于两种不同的g蛋白偶联食欲素受体（OX-R1和OX-R2），它们来源于不同的基因，在整个中枢神经系统中表达不同。在本研究中，我们使用多克隆抗肽抗血清详细分析了ox - r1免疫反应神经元在大鼠下丘脑的分布。为了确定下丘脑中食欲素作用的化学介质，根据先前显示的影响摄食和饮酒行为的肽含量，对含有ox - r1的神经元进行了表征。具有OX-R1免疫反应性的神经元广泛分布于下丘脑，胞体位于视交叉上、脑室周围、室旁（包括大细胞分裂和旁细胞分裂）、视上核、弓形核、腹内侧核、背内侧核和结节乳头核以及下丘脑外侧区。在室旁核和视上核的大细胞神经元中，OX-R1免疫反应在加压素和催产素含神经元中均可见。OX-R1在视交叉上核的血管加压素和血管活性肠多肽（VIP）神经元、室旁核的生长抑素神经元和室旁核的促肾上腺皮质激素释放激素（CRH）神经元中表现出免疫反应性。在弓形核中，OX-R1免疫反应性存在于腹内侧神经肽Y （NPY）和针刺相关肽（AGRP）神经元以及腹外侧proopiornelanocortin （POMC）和可卡因和安非他明调节转录物（CART）神经元中。在下丘脑外侧区，OX-R1在黑色素浓缩激素（MCH）和含食欲素的神经元中表现出免疫反应性。在下丘脑结节乳头核中，许多含组胺的神经元显示ox - r1免疫反应性。研究结果支持了食欲素对控制食物摄入和体液平衡的下丘脑神经元有重要作用的观点，但食欲素也可能调节其他神经内分泌系统。
The neuropeptides orexin-A and orexin-B are produced in neurons of the lateral hypothalamic area and have been implicated to be involved in the regulation of food/water intake and sleep-wake control. The orexins act at two different G-protein-coupled orexin receptors (OX-R1 and OX-R2) that are derived from separate genes and expressed differentially throughout the central nervous system. In the present study, we have used a polyclonal antipeptide antiserum to analyse in detail the distribution of OX-R1-immunoreactive neurons in the rat hypothalamus. In order to identify the chemical mediators of orexin action in the hypothalamus, the OX-R1-containing neurons were characterized with regard to the content of peptides shown previously to affect ingestive and drinking behaviour. Neurons containing OX-R1 immunoreactivity were widely distributed in the hypothalamus with cell bodies located in the suprachiasmatic, periventricular, paraventricular (both magno- and parvocellular division), supraoptic, arcuate, ventromedial, dorsomedial and tuberomammillary nuclei and the lateral hypothalamic area. In magnocellular neurons of the paraventricular and supraoptic nuclei, OX-R1 immunoreactivity was seen in both vasopressin- and oxytocin-containing neurons. OX-R1 immunoreactivity was demonstrated in vasopressin and vasoactive intestinal polypeptide (VIP) neurons of the suprachiasmatic nucleus, in somatostatin neurons of the periventricular nucleus and in corticotropin-releasing hormone (CRH) neurons of the parvocellular paraventricular nucleus. In the arcuate nucleus, OX-R1 immunoreactivity was present in neuropeptide Y (NPY) and agouti-related peptide (AGRP) neurons of the ventromedial part as well as in proopiornelanocortin (POMC) and cocaine- and amphetamine-regulated transcript (CART) neurons of the ventrolateral division. In the lateral hypothalamic area, OX-R1 immunoreactivity was demonstrated in melanin-concentrating hormone (MCH)- and orexin-containing neurons. In the hypothalamic tuberomammillary nucleus, OX-R1-immunoreactivity was shown in many histamine-containing neurons. The results support the idea that orexins have important actions on hypothalamic neurons that control food intake and fluid balance, but also that orexins may regulate other neuroendocrine systems.