An epithelial niche in the Drosophila Ovary undergoes long-range stem cell replacement
An epithelial niche in the Drosophila Ovary undergoes long-range stem cell replacement
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DOI：
10.1016/j.stem.2007.07.009
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发表时间：
2007-09-01
期刊：
影响因子：
23.9
通讯作者：
Spradling, Allan
中科院分区：
文献类型：
--
作者：
Nystul, Todd;Spradling, Allan
成体上皮干细胞被认为存在于特定的利基环境中，在那里它们通过与基质细胞的黏附和细胞间信号来维持。在含有多个相邻干细胞的壁龛中，例如那些维持果蝇生殖细胞的干细胞，丢失的干细胞被邻近干细胞的分裂或转运细胞的逆转所取代。我们已经将果蝇毛囊干细胞(FSC)生态位描述为上皮生态位的模型，以了解不相邻的细胞是否也可以产生干细胞替代。在果蝇卵巢的相对边缘，正好有两个无基质的FSC壁龛放置在固定的位置上。FSC子代在增殖并形成卵泡细胞单层之前，会有规律地跨越卵巢的宽度迁移到另一个壁龛。跨国移民的FSC女儿与常驻的FSC争夺生态位，是替换FSC的来源。干细胞子体靶向遥远的小生境并取代其常驻干细胞的能力表明，癌前突变可能会在以干细胞为基础的组织中从一个小生境传播到另一个小生境。
Adult epithelial stem cells are thought to reside in specific niches, where they are maintained by adhesion to stromal cells and by intercellular signals. In niches that harbor multiple adjacent stem cells, such as those maintaining Drosophila germ cells, lost stem cells are replaced by division of neighboring stem cells or reversion of transit cells. We have characterized the Drosophila follicle stem cell (FSC) niche as a model of the epithelial niche to learn whether nonneighboring cells can also generate stem cell replacements. Exactly two stroma-free FSC niches holding single FSCs are located in fixed locations on opposite edges of the Drosophila ovariole. FSC daughters regularly migrate across the width of the ovariole to the other niche before proliferating and contributing to the follicle cell monolayer. Crossmigrating FSC daughters compete with the resident FSC for niche occupancy and are the source of replacement FSCs. The ability of stem cell daughters to target a distant niche and displace its resident stem cell suggests that precancerous mutations might spread from niche to niche within stem cell-based tissues.