Melatonin receptor subtype expression in human cerebellum
Melatonin receptor subtype expression in human cerebellum
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DOI：
10.1097/00001756-199812210-00011
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发表时间：
1998-12-21
期刊：
影响因子：
1.7
通讯作者：
Dubocovich, ML
中科院分区：
文献类型：
--
作者：
Al-Ghoul, WM;Herman, MD;Dubocovich, ML
我们报道了哺乳动物MT（1）（Mel（1a））和MT 2（Mel（1b））（1）褪黑素受体亚型在人类小脑皮质中的不同细胞分布。非选择性放射性配体2-[I-125] iodomelatonin的外分子层的特异性结合显着高于颗粒细胞层。褪黑激素受体亚型的表达进行了评估原位杂交使用选择性和特异性地高辛标记的反义寡核苷酸探针。这是首次证明MT 2褪黑素受体mRNA在人小脑Bergmann胶质细胞和星形胶质细胞中的表达。另一方面，MT（1）褪黑素受体mRNA在篮状星状细胞和颗粒细胞中均有表达。我们的结论是MT（1）和MT 2褪黑激素受体在人类小脑皮质的异质性表达。(C)1998年利平科特·威廉姆斯和威尔金斯。
WE report the distinct cellular distribution in the human cerebellar cortex of the mammalian mt(1) (Mel(1a)) and MT2 (Mel(1b)) (1) melatonin receptor subtypes. Specific binding of the non-selective radioligand 2-[I-125]iodomelatonin to the outer molecular layer was significantly higher than to the granule cell layer. Melatonin receptor subtype expression was assessed by in situ hybridization using selective and specific digoxigenin-labeled antisense oligonucleotide probes. This is the first demonstration of MT2 melatonin receptor mRNA expression in human cerebellar Bergmann glia and astrocytes. On the other hand, the mt(1) melatonin receptor mRNA was expressed in both basket-stellate cells and granule cells. We conclude that mt(1) and MT2 melatonin receptors are heterogeneously expressed in human cerebellar cortex. (C) 1998 Lippincott Williams & Wilkins.