ROLE OF PLATELETS IN SMOOTH-MUSCLE CELL-PROLIFERATION AND MIGRATION AFTER VASCULAR INJURY IN RAT CAROTID-ARTERY
ROLE OF PLATELETS IN SMOOTH-MUSCLE CELL-PROLIFERATION AND MIGRATION AFTER VASCULAR INJURY IN RAT CAROTID-ARTERY
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DOI：
10.1073/pnas.86.21.8412
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发表时间：
1989-11-01
影响因子：
11.1
通讯作者：
REIDY, MA
中科院分区：
文献类型：
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作者：
FINGERLE, J;JOHNSON, R;REIDY, MA
在通过单次ip使大鼠血小板减少的大鼠中研究内膜损伤形成。注射抗大鼠血小板的多克隆抗体，使循环血小板计数降至正常值的1%以下。然后用2French球囊导管剥脱颈动脉的内皮，之后未发现血小板粘附于暴露的内皮下。在对照组动物中，血小板立即粘附在裸露的动脉上。剥脱6小时后，鸟氨酸脱羧酶的mRNA，早期G1事件的标志物，被发现在两个动脉和控制动脉升高。损伤后两天，发现与未损伤的颈动脉相比，动脉减少大鼠中的平滑肌细胞复制率显著升高（13.7% ± 0.1%）。8.4%对0.65%。0.23%），但与用非免疫IgG处理的动物的裸露颈动脉中观察到的复制率相似。这些动物之间的一个重要差异是，在第4天，在血小板减少的动物中没有观察到内膜增厚，到第7天，内膜仍然显著小于对照组大鼠的内膜。在整个实验中，在单独的一组动物中，在球囊导管损伤后7天未观察到内膜损伤。从这些结果中，我们得出结论，血小板不发挥作用，在启动平滑肌细胞增殖损伤后球囊导管，但可能会调节他们的运动到内膜。
Intimal lesion formation was investigated in rats made thrombocytopenic by a single ip. injection of a polyclonal antibody made against rat platelets that reduced circulating platelet counts to less than 1% of normal. The carotid artery was then denuded of endothelium with a 2 French balloon catheter, after which no platelets were found adhering to the exposed subendothelium. In control animals, platelets adhered instantly to the denuded artery. Six hours after denudation mRNA for ornithine decarboxylase, a marker for early G1 events, was found to be elevated in both thrombocytopenic and control arteries. Two days after injury the smooth muscle cell replication rate in thrombocytopenic rats was found to be significantly elevated as compared with that in uninjured carotids (13.7% .+-. 8.4% vs 0.65% .+-. 0.23%) but was similar to the replication rate observed in denuded carotid arteries from animals treated with nonimmune IgG. One important difference between these animals was that no intimal thickening was observed in thrombocytopenic animals at day 4, and by day 7 the intimas were still significantly smaller than those from control rats. In a separate group of animals which were thrombocytopenic for the entire experiment, no intimal lesions were observed 7 days after injury by balloon catheter. From these results, we conclude that platelets do not play a role in the initiation of smooth muscle cell proliferation after injury by balloon catheter but may regulate their movement into the intima.