A structural model for the alpha-subunit of transducin. Implications of its role as a molecular switch in the visual signal transduction mechanism.
A structural model for the alpha-subunit of transducin. Implications of its role as a molecular switch in the visual signal transduction mechanism.
复制标题
转导蛋白α亚基的结构模型。
DOI：
10.1016/0014-5793(87)80867-0
复制
发表时间：
1987
期刊：
影响因子：
3.5
通讯作者：
Ho,YK
中科院分区：
文献类型：
--
作者：
Hingorani,VN;Ho,YK
Transducin是一种GTP结合蛋白，介导从光解视紫红质到cGMP磷酸二酯酶的光激活信号，在视觉激发过程中起关键作用。生物化学研究表明，transducin的Tα亚基由三个功能结构域组成，一个用于视紫红质/Tβγ相互作用，另一个用于鸟嘌呤核苷酸结合，第三个用于激活磷酸二酯酶。将Tα的一级序列沿着与二级结构、亲水性和折叠拓扑预测相结合，并与同源蛋白质进行比较，从而构建了Tα亚基的三维模型。在此基础上提出了Tα偶联作用的分子机理。
Transducin is a GTP‐binding protein which mediates the light activation signal from photolyzed rhodopsin to cGMP phosphodiesterase and is pivotal in the visual excitation process. Biochemical studies suggest that the Tαsubunit of transducin is composed of three functional domains, one for rhodopsin/Tβγinteraction, another for guanine nucleotide binding, and a third for the activation of phosphodiesterase. The integration of the primary sequence of Tαalong with secondary structure, hydropathy and folding topology predictions, and a comparison with homologous proteins have led to the construction of a three‐dimensional model of the Tαsubunit. A molecular mechanism which underlies the coupling action of Tαis suggested on the basis of this model.