GENES FOR EXTRACELLULAR MATRIX-DEGRADING METALLOPROTEINASES AND THEIR INHIBITOR, TIMP, ARE EXPRESSED DURING EARLY MAMMALIAN DEVELOPMENT
GENES FOR EXTRACELLULAR MATRIX-DEGRADING METALLOPROTEINASES AND THEIR INHIBITOR, TIMP, ARE EXPRESSED DURING EARLY MAMMALIAN DEVELOPMENT
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DOI：
10.1101/gad.3.6.848
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发表时间：
1989-06-01
影响因子：
10.5
通讯作者：
WERB, Z
中科院分区：
文献类型：
--
作者：
BRENNER, CA;ADLER, RR;WERB, Z
在哺乳动物胚胎发育过程中，细胞外基质（ECM）重塑伴随着细胞迁移、细胞-细胞相互作用、胚胎扩张、子宫着床和组织侵入。我们已经发现，小鼠胚胎分泌功能性ECM降解金属蛋白酶，包括胶原酶和基质溶解素，这是由金属蛋白酶的组织抑制剂（TIMP），并在围植入期发育和内胚层分化调节。胶原酶，基质分解素，和TIMP的mRNA转录被检测为母体转录在未受精卵，存在于受精卵和卵裂阶段，并增加在囊胚阶段和内胚层分化。这些数据表明，在哺乳动物胚胎的生长，发育和植入过程中，金属蛋白酶在细胞-ECM相互作用中发挥作用。
Extracellular matrix (ECM) remodeling accompanies cell migration, cell-cell interactions, embryo expansion, uterine implantation, and tissue invasion during mammalian embryogenesis. We have found that mouse embryos secrete functional ECM-degrading metalloproteinases, including collagenase and stromelysin, that are inhibitable by the tissue inhibitor of metalloproteinases (TIMP) and that are regulated during peri-implantation development and endoderm differentiation. mRNA transcripts for collagenase, stromelysin, and TIMP were detected as maternal transcripts in the unfertilized egg, were present at the zygote and cleavage stages, and increased at the blastocyst stage and with endoderm differentiation. These data suggest that metalloproteinases function in cell-ECM interactions during growth, development, and implantation of mammalian embryos.