Characterization of anti-inflammatory effect and possible mechanism of action of Tibouchina granulosa
Characterization of anti-inflammatory effect and possible mechanism of action of Tibouchina granulosa
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DOI：
10.1111/jphp.12712
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发表时间：
2017-06-01
影响因子：
3.3
通讯作者：
Fernandes, Patricia D.
中科院分区：
文献类型：
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作者：
Sobrinho, Andrea P.;Minho, Alan S.;Fernandes, Patricia D.
目的粉刺粉刺，俗称Quaresmeira‘，是传统医学中广泛使用的一种植物，从其叶子中提取提取液作为冲剂。蒂布瓦属的其他物种被用作抗菌、抗氧化剂或抗利什曼药。本研究旨在探讨颗粒菌在急性炎症模型中的生物学作用。方法化学分析表明其含有原花青素和黄酮类化合物。给小鼠灌胃1、3、10、30或100 mg/kg的颗粒毛滴虫叶液，通过福尔马林诱导的舔血反应、抑制角叉菜胶诱导的细胞向皮下气囊(SAP)的迁移以及抑制从SAP收集的炎性渗出物中炎症介质的产生来评价其抗炎作用。各剂量组均能抑制福尔马林诱导的第一相和第二相的舔食反应。结论综合考虑，细叶草叶具有抗炎作用，可用于新型植物药物的制备。
ObjectivesTibouchina granulosa, popularly known as quaresmeira', belong to a genus widely used in the traditional medicine as infusions from their leaves. Other species of Tibouchina are used as antibacterial, antioxidant or antileishmanial. In this work, our objectives were to investigate the biological effects of T. granulosa in models of acute inflammation.MethodsChemical analysis showed the presence of proanthocyanidins and flavonoids. Infusions from leaves of T. granulosa (1, 3, 10, 30 or 100 mg/kg) were orally administered to mice, and the anti-inflammatory effects were evaluated by the formalin-induced licking response, inhibition of carrageenan-induced cell migration into subcutaneous air pouch (SAP) and inhibition of inflammatory mediator production in inflammatory exudate collected from SAP.Key findingsOur data indicate that tested doses of T. granulosa infusion reduced cell migration, protein extravasated to SAP and cytokine production (i.e. TNF- and IL-10). All doses also inhibited the first and second phase of formalin-induced licking response.ConclusionsTaken together, our results indicate that leaves of T. granulosa present anti-inflammatory effect and can be useful in the preparation of new phytomedicines.