High-performance thin-layer chromatographic method for the fluorescence detection of three nitroimidazole residues in pork and poultry tissue.
High-performance thin-layer chromatographic method for the fluorescence detection of three nitroimidazole residues in pork and poultry tissue.
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DOI：
10.1016/0021-9673(96)00101-x
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发表时间：
1996-06
期刊：
影响因子：
--
通讯作者：
M. Gaugain;J. Abjean
中科院分区：
文献类型：
--
作者：
M. Gaugain;J. Abjean
建立了猪肉和禽肉中硝硝唑、咪甲咪唑及其主要代谢物羟基二甲基咪唑的高效薄层荧光检测方法。硝基咪唑经二氯甲烷萃取、蒸发后，在醋酸铵缓冲液中重新溶解。缓冲相用正己烷洗涤。样品用固相萃取净化，洗脱液蒸发。最后的提取物再悬浮在甲醇中，然后在HPTLC板上斑点。用甲醇和乙酸乙酯多次显影后，干燥，喷洒吡啶，在紫外箱(312 Nm)上观察。方法检出限分别为2μg/kg、5μg/kg和5μg/kg。验证剂量分别为10μg/kg、5μg/kg和5μg/kg。
A high-performance thin-layer chromatographic method with fluorescence detection was developed for the qualitative determination of ronidazole, dimetridazole and their major metabolite, hydroxydimetridazole, in pork and poultry muscle. After extraction with dichloromethane and evaporation, the nitroimidazoles are redissolved in ammonium acetate buffer. The buffer phase is washed with hexane. The sample is cleaned-up by solid-phase extraction and the eluate evaporated. The final extract is resuspended in methanol and then spotted on an HPTLC plate. After multiple development with methanol and ethylacetate, the plate is dried, sprayed with pyridine and observed on an UV box (312 nm). The detection limits of this method are about 2 μg/kg for ronidazole, 5 μg/kg for dimetridazole and less than 5 μg/kg for hydroxydimetridazole. Validation was performed to levels of 10 μg/kg for dimetridazole, 5 μg/kg for ronidazole and 5 μg/kg for hydroxydimetridazole.