Discovery of ligands for a novel target, the human telomerase RNA, based on flexible-target virtual screening and NMR.
Discovery of ligands for a novel target, the human telomerase RNA, based on flexible-target virtual screening and NMR.
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基于灵活靶点虚拟筛选和 NMR，发现新靶点——人端粒酶 RNA 的配体。
DOI：
10.1021/jm800825n
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发表时间：
2008
影响因子：
7.3
通讯作者：
James,ThomasL
中科院分区：
文献类型：
--
作者：
Pinto,IreneGómez;Guilbert,Christophe;Ulyanov,NikolaiB;Stearns,Jay;James,ThomasL
The human ribonucleoprotein telomerase is a validated anticancer drug target, and hTR-P2b is a part of the human telomerase RNA (hTR) essential for its activity. Interesting ligands that bind hTR-P2b were identified by iteratively using a tandem structure-based approach: docking of potential ligands from small databases to hTR-P2b via the program MORDOR, which permits flexibility in both ligand and target, with subsequent NMR screening of high-ranking compounds. A high percentage of the compounds tested experimentally were found via NMR to bind to the U-rich region of hTR-P2b; most have MW < 500 Da and are from different compound classes, and several possess a charge of 0 or +1. Of the 48 ligands identified, 24 exhibit a decided preference to bind hTR-P2b RNA rather than A-site rRNA and 10 do not bind A-site rRNA at all. Binding affinity was measured by monitoring RNA imino proton resonances for some of the compounds that showed hTR binding preference.
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对拉伸诱发肌电反应的传入贡献。
影响因子：
2.5
作者：
R. Jaeger;G. Gottlieb;G. Agarwal;A. Tahmoush
通讯作者：
A. Tahmoush
肌肉振动对正常人和运动障碍患者的影响
DOI：
10.1159/000394158
发表时间：
1973
影响因子：
2.2
作者：
K. Hagbarth
通讯作者：
K. Hagbarth
选择性充分激活来自肌梭和高尔基腱器官的大传入神经。
DOI：
10.1111/j.1748-1716.1960.tb01939.x
发表时间：
1960
期刊：
Acta physiologica Scandinavica
影响因子：
--
作者：
A. Lundberg;G. Winsbury
通讯作者：
G. Winsbury
影响因子：
5.3
作者：
ELDRED, E;LINDSLEY, DF;BUCHWALD, JS
通讯作者：
BUCHWALD, JS
影响因子：
--
作者：
P. Fowler;A. Guz
通讯作者：
A. Guz