Mayaro virus fever in French Guiana:: Isolation, identification, and seroprevalence
Mayaro virus fever in French Guiana:: Isolation, identification, and seroprevalence
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DOI：
10.4269/ajtmh.1998.59.452
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发表时间：
1998-09-01
影响因子：
3.3
通讯作者：
Sarthou, JL
中科院分区：
文献类型：
--
作者：
Talarmin, A;Chandler, LJ;Sarthou, JL
本文报道首次从法属圭亚那的一名病人中分离到马亚罗病毒。最初使用特异性小鼠抗体的免疫荧光抗体检测进行鉴定，并通过空斑减少中和试验和逆转录-聚合酶链反应进行确认。为了确定MAY病毒感染是否在法属圭亚那广泛存在，进行了血清学调查，以确定法属圭亚那各种族群体和地区对该病毒的抗体流行情况。使用血凝抑制（I-EI）试验筛选人血清（n = 1，962）。为了确定MAY病毒是否在热带雨林中传播，在猴子群体中进行了血清调查。还使用HI试验筛选猴血清（n = 150）的MAY病毒抗体。在检测的人血清中，6.3%的人血清抗MAY病毒抗体呈阳性。不同年龄组之间的MAY病毒血清阳性率存在显著差异。血清阳性率随着年龄的增长而增加，10-19岁的人与10岁以下的人相比有很大的增加。在对年龄进行调整后，还发现居住地之间存在显著差异。在与森林接触的人群中，抗MAY病毒抗体的流行率较高，尤其是在Haut Oyapock地区（比值比[OR] = 97.7，95%置信区间[CT] = 48.2-197.9）和沿着马罗尼河（OR = 39.7，95% CI = 20.6-76.6）。这项研究中观察到的种族差异可能是由于居住地的差异。在猴中，老年Alouatta（66.0%）的血清阳性率高于Saguinus midas（18.2%）。在Alouatta中，血清阳性率随体重和年龄而显著增加。这项研究表明，MAY病毒存在于法属圭亚那，人类感染发生在人们居住的热带雨林附近地区。
This paper reports the first isolation of Mayaro (MAY) virus from a patient infected in French Guiana. The identification was initially performed using immunofluorescent antibody testing with specific mouse antibody, and confirmed by plaque-reduction neutralization testing and reverse transcription-polymerase chain reaction. To determine if MAY virus infection is widespread in French Guiana, a serosurvey was performed to determine the prevalence of antibody to this virus in various ethnic groups and areas of French Guiana. Human sera (n = 1,962) were screened using the hemagglutination inhibition (I-EI) test. To determine whether MAY virus circulates in the rain forest, a serosurvey in monkey populations was performed. Monkey sera (n = 150) were also screened for antibody to MAY virus using HI testing. Of the human sera tested, 6.3% were positive for anti-MAY virus antibodies. Significant differences in MAY virus seroprevalence between different age groups were observed. Seroprevalence rates increased with age, with a large increase in people 10-19 years of age in comparison with those less than 10 years of age. After adjustment for age, significant differences were also found between places of residence. The prevalence of anti-MAY virus antibody was higher in people living in contact with the forest, especially in the Haut Oyapock area (odds ratio [OR] = 97.7, 95% confidence interval [CT] = 48.2-197.9) and along the Maroni River (OR = 39.7, 95% CI = 20.6-76.6). The ethnic differences observed in this study were probably due to differences in residence. Among monkeys, higher seroprevalence rates were found in Alouatta seniculus (66.0%) than in Saguinus midas (18.2%). Among Alouatta, the seroprevalence increased significantly with weight land therefore with age). This study indicates that MAY virus is present in French Guiana, and human infections occur in areas where people live near the tropical rain forest.