Reflection spectrophotometric measurement of O2 uptake in pulmonary arterioles of cats.
Reflection spectrophotometric measurement of O2 uptake in pulmonary arterioles of cats.
复制标题
反射分光光度法测量猫肺小动脉中 O2 的吸收。
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
N. Staub
中科院分区：
文献类型：
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作者：
R. Conhaim;N. Staub
我们试图从实验和理论上确定是否有大量的氧气在到达肺泡壁毛细血管之前进入肺血。我们建立了一个显微分光光度计，使我们能够通过从直径小至100微米的横切冷冻肺动脉反射的光来记录血红蛋白饱和度值。我们准备了麻醉猫，为毛细前氧合提供最佳条件，用冷冻液体丙烷快速冷冻它们的肺，并切除这些肺。我们用显微分光光度计检查了肺横截面，并记录了表明毛细血管前氧合存在的数据。这些数据并没有告诉我们这个过程通常对肺氧合的贡献有多大，因为丙烷冻结肺部的速度不够快，无法完全捕捉到它们在生命中的情况。因此，我们利用肺动脉几何形状和弥散能力的可用数据进行了一些计算来估计毛细前氧合的程度。我们的结论是，在静止状态下，肺动脉血液的含氧率可能高达15%，而在氧气呼吸时，其含氧率可能高达100%。
We have attempted to determine both experimentally and theoretically whether a significant quantity of oxygen enters the pulmonary blood before it reaches the alveolar wall capillaries. We built a microspectrophotometer that allowed us to record oxyhemoglobin saturation values with light reflected from transected frozen pulmonary arteries as small as 100 microns in diameter. We prepared anesthetized cats to provide optimal conditions for precapillary oxygenation, quickly froze their lungs with chilled liquid propane, and removed these lungs. We examined lung cross sections with the microspectrophotometer and recorded data indicating the presence of precapillary oxygenation. These data did not tell us how much this process normally contributes to total lung oxygenation because propane did not freeze the lungs fast enough to capture conditions exactly as they are in life. We therefore made several calculations to estimate the extent of precapillary oxygenation using available data on pulmonary arterial geometry and diffusing capacity. We concluded that pulmonary arterial blood may be as much as 15% oxygenated by this process at rest and as much as 100% during oxygen breathing.