Histologic effects of external ultrasound-assisted lipectomy on adipose tissue
Histologic effects of external ultrasound-assisted lipectomy on adipose tissue
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DOI：
10.1007/s00266-007-9031-8
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发表时间：
2008-01-01
影响因子：
2.4
通讯作者：
D'Andrea, Francesco
中科院分区：
文献类型：
--
作者：
Ferraro, Giuseppe A.;De Francesco, Francesco;D'Andrea, Francesco
本研究旨在观察不同频率的超声波对腹部脂肪组织的影响。通过组织学和免疫组织化学检查进行外部超声辅助脂肪切除术（XUAL），用于评估脂肪组织的改变，包括细胞和胶原纤维。免疫荧光水平的结果表明，使用频率为 1 MHz、功率为 3 W 的超声波不会导致任何改变或仅导致有限的细胞破坏，而胶原纤维完好无损。相反，当超声频率为 2 MHz，特别是 3 MHz 时，脂肪细胞的变化通常很明显。观察到大量脂肪组织破坏，经油红-O 染色证实。此外，在免疫荧光水平上，检测到弥漫性胶原纤维回缩。与完整对照样本的活检相比，这一点尤其明显，后者显示正常的脂肪组织和完整的胶原纤维。使用形态学技术获得的结果（不允许固定伪影并包括胶原蛋白观察）表明，使用 XUAL 技术，1 MHz 超声波不会引起细胞改变。相比之下，2 MHz 和 3 MHz 频率都能够导致脂肪组织完全破坏，包括脂肪细胞和胶原纤维的破坏。
This study aimed to observe the effects of ultrasound waves at different frequencies on abdominal fat tissue. External ultrasound-assisted lipectomy (XUAL) via both histologic and immunohistochemic examinations was used to assess adipose tissue alterations, including cells and collagenic fibers. The results, at the immunofluorescence level, show that ultrasound used at 1 MHz with a potency of 3 W resulted in no alterations or only limited cell destruction with collagen fibers intact. In contrast, when the ultrasound was 2 and especially 3 MHz, adipocyte alterations usually were evident. Massive adipose tissue destruction, confirmed using Oil red-O staining, was observed. In addition, at the immunofluorescence level, diffuse collagen fiber retraction was detected. This was particularly evident in comparisons with biopsies of intact control samples, which showed normal adipose tissue and intact collagen fibers. The results obtained using morphologic techniques, which do not allow fixation artifacts and include collagen observations, demonstrate that with the XUAL technique, ultrasound at 1 MHz does not induce cellular alterations. In contrast, both 2- and 3-MHz frequencies are capable of causing complete fat tissue disruption, including destruction of adipose cells and collagenic fibers.