Leaf cuticular waxes are arranged in chemically and mechanically distinct layers:: evidence from Prunus laurocerasus L.
Leaf cuticular waxes are arranged in chemically and mechanically distinct layers:: evidence from Prunus laurocerasus L.
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DOI：
10.1046/j.1365-3040.2000.00581.x
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发表时间：
2000-06-01
影响因子：
7.3
通讯作者：
Riederer, M
中科院分区：
文献类型：
--
作者：
Jetter, R;Schäffer, S;Riederer, M
研究了月桂酸樱桃叶片表皮蜡质的组成和空间排列。在蜡混合物中鉴定出三萜类化合物乌索酸和油酸以及烷烃、脂肪酸、醛、伯醇和醇乙酸酯。上、下叶表面的萃取量分别为280和830 mg m（-2）。从角质层的最外层的蜡的机械去除协议进行了设计和评估。用这些方法中选择性最强的方法，在达到尖锐的、物理上有抵抗力的边界之前，可以从近轴面除去130 mg m（-2）的表皮蜡。这样获得的化合物被解释为“表皮蜡”，因为它们位于角质基质表面上的独特层中。可以将其转移到玻璃上并通过扫描电子显微镜观察，月桂酸樱桃的表皮蜡完全由脂肪族化合物组成，而剩余的表皮内蜡包含63%的三萜类化合物。这种层状结构的生态相关性识别的植物营养型真菌和草食性昆虫，探测表面组成的信号刺激进行了讨论。
The composition and spatial arrangement of cuticular waxes on the leaves of Prunus laurocerasus were investigated. In the wax mixture, the triterpenoids ursolic acid and oleanolic acid as well as alkanes, fatty acids, aldehydes, primary alcohols and alcohol acetates were identified, The surface extraction of upper and lower leaf surfaces yielded 280 mg m(-2) and 830 mg m(-2), respectively. Protocols for the mechanical removal of waxes from the outermost layers of the cuticle were devised and evaluated. With the most selective of these methods, 130 mg m(-2) of cuticular waxes could be removed from the adaxial surface before a sharp, physically resistant boundary was reached, Compounds thus obtained are interpreted as 'epicuticular waxes' with respect to their localization in a distinct layer on the surface of the cutin matrix. The epicuticular wax him can be transferred onto glass and visualized by scanning electron microscopy, Prunus laurocerasus epicuticular waxes consisted entirely of aliphatic compounds, whereas the remaining intracuticular waxes comprised 63% of triterpenoids. The ecological relevance of this layered structure for recognition by phytotrophic fungi and herbivorous insects that probe the surface composition for sign stimuli is discussed.