Characterization of a new lignin peroxidase gene (GLG6) from Phanerochaete chrysosporium.
Characterization of a new lignin peroxidase gene (GLG6) from Phanerochaete chrysosporium.
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来自金孢原毛平革菌的新木质素过氧化物酶基因 (GLG6) 的表征。
DOI：
10.1016/s0006-291x(05)80884-8
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发表时间：
1990
影响因子：
3.1
通讯作者：
Reddy,CA
中科院分区：
文献类型：
--
作者：
Naidu,PS;Zhang,YZ;Reddy,CA
对黄孢原毛平革菌（P. chrysosporium）新的木质素过氧化物酶（LIP）基因GLG 6的序列分析表明，该基因编码一个成熟的LIP蛋白，预测Mr为36，454。成熟蛋白前有一个28个氨基酸的信号肽。GLG 6的编码区被9个50-57 bp的内含子所打断，其编码的LIP与LIP同工酶H2、H3和H10的同源性分别为72%、88%和82%。将GLG 6编码的LIP的N端序列与LIP蛋白H2、H8、H10的N端序列进行比较也表明，GLG 6编码的LIP与H2蛋白的相关性比与H8和H10蛋白的相关性相对较低。GLG 6的表达与其他LIP基因相似，仅发生在次级代谢过程中。
Sequence analysis of a new lignin peroxidase (LIP) gene,GLG6, fromP.chrysosporiumshowed that it encodes a mature LIP protein with a predictedMrof 36,454. A 28 aa signal peptide precedes the mature protein. The coding region ofGLG6is interrupted by nine introns ranging in size from 50–57 bp.GLG6encoded-LIP has 72%, 88% and 82% homology, respectively, to the LIP isozymes H2, H3, and H10. Comparison of theN-terminal sequence ofGLG6-encoded LIP to that of the LIP proteins H2, H8, H10 also showed that the former is relatively less related to the H2 protein than it is to the H8 and H10 proteins. Expression ofGLG6, similar to the otherLIPgenes, occurs only during secondary metabolism.