Investigation of copolymers of styrene and ethyl methacrylate by size-exclusion chromatography and gradient HPLC
Investigation of copolymers of styrene and ethyl methacrylate by size-exclusion chromatography and gradient HPLC
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通过尺寸排阻色谱法和梯度 HPLC 研究苯乙烯和甲基丙烯酸乙酯的共聚物
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
W. Wunderlich
中科院分区：
文献类型：
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作者：
G. Glöckner;M. Stickler;W. Wunderlich
摘要用反相色谱在C18柱上按组成分离了苯乙烯和甲基丙烯酸乙酯(SEMA)共聚物。起始洗脱剂为甲醇，共聚物不溶于水，加入四氢呋喃形成洗脱梯度，四氢呋喃的极性比甲醇小，但对SEMA共聚物来说是很好的溶剂。将该方法应用于模型混合物的尺寸排阻色谱分离，得到了比正相模式下使用递增极性梯度的极性柱更好的分离效果。
SummaryCopolymers of styrene and ethyl methacrylate (SEMA) have been separated according to composition by reversed-phase chromatography on C18 columns. The starting eluent was methanol, in which the copolymers are insoluble, and an elution gradient was formed by the addition of tetrahydrofuran, which is less polar than methanol yet a good solvent for SEMA copolymers. The method was applied to fractions obtained by size exclusion chromatography of model mixtures, and yielded a separation superior to that obtained in normal-phase mode on polar columns using gradients of increasing polarity.