Isolation and characterization of 13 polymorphic microsatellite loci for the fig wasp, Ceratosolen sp. (Hymenoptera: Agaonidae)
Isolation and characterization of 13 polymorphic microsatellite loci for the fig wasp, Ceratosolen sp. (Hymenoptera: Agaonidae)
复制标题
DOI：
10.1007/s13355-015-0387-y
复制
发表时间：
2016-01
影响因子：
1.3
通讯作者：
Lu Tan;Jun‐Yin Deng;Ronghua Fu;Yan Chen
中科院分区：
文献类型：
--
作者：
Lu Tan;Jun‐Yin Deng;Ronghua Fu;Yan Chen
无花果黄蜂寿命短，脆弱，飞行能力弱。它们的远距离传播高度依赖于树冠上方的气流。寄主榕属物种的生命形式决定了它们的释放地点，因此也决定了它们进入树冠上方快速流动空气的能力。热带榕属植物tikouaBur。是一种雌雄异株匍匐灌木，部分埋有无花果。其繁殖系统和生长形态与传粉榕小蜂种群间有限的传播能力和高度的遗传分化是一致的。高度多态的微卫星位点将允许检测其传粉者的精细遗传结构，并阐明其与宿主的共同进化动力学。我们为角藻找到了13个多态微卫星位点。（Agaonidae），专性授粉榕蜂。tikoua。每个引物在中国西南3个群体中进行了有效性和多态性测试。在任何位点对之间未检测到明显的连锁不平衡。总体而言，每个位点的等位基因数在2 ~ 17之间。在群体水平上，观察杂合度为0 ~ 0.833，期望杂合度为0 ~ 0.840。这些多态性位点将成为筛选传粉媒介ofF遗传变异的有用工具。与宿主有关的变异。
Fig wasps are short-lived, delicate and weak-flying. Their long-distance dispersal is highly dependent on the air flow above the canopy. The life forms of their hostFicusspecies determine their place of release, and so their ability of entering the fast-flowing air above the canopy.Ficus tikouaBur. is a dioecious prostrate shrub with partially buried figs. Its breeding system and growth form are consistent with limited dispersal ability and high genetic differentiation between populations of its pollinating fig wasps. Highly polymorphic microsatellite loci will allow detection of fine-scale genetic structure in its pollinator, and elucidate co-evolutionary dynamics with its host. We developed 13 polymorphic microsatellite loci forCeratosolensp. (Agaonidae), the obligate pollinating fig wasp ofF. tikoua. The validity and polymorphism of each primer was tested in three populations from Southwest China. No significant linkage disequilibrium was detected between any locus pairs. Overall, allele numbers per locus ranged from 2 to 17. At population level, the observed and expected heterozygosities ranged from 0 to 0.833 and from 0 to 0.840, respectively. These polymorphic loci will be a helpful tool for screening genetic variation within the pollinator ofF. tikouaand relating variation to that of its host.