CHARACTERISTICS OF HUMAN PERIODONTAL-LIGAMENT CELLS-INVITRO
CHARACTERISTICS OF HUMAN PERIODONTAL-LIGAMENT CELLS-INVITRO
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DOI：
10.1016/0003-9969(90)90062-f
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发表时间：
1990-01-01
影响因子：
3
通讯作者：
SAUK, JJ
中科院分区：
文献类型：
--
作者：
SOMERMAN, MJ;YOUNG, MF;SAUK, JJ
牙周膜细胞可能在牙周硬组织和软组织的调节中起作用，但其具体功能尚未确定。为了进一步评估它们在牙周动态平衡中的作用，对它们进行了成骨细胞样行为的检测；在体外没有发现典型的成骨细胞反应。牙周韧带细胞产生PGE2和异丙肾上腺素介导的cAMP反应，但对降钙素或甲状旁腺素没有类似的反应方式。当暴露在PGE2、异丙肾上腺素或1，25(OH)2维生素D3中时，通过[35S]-蛋氨酸掺入测定，它们的蛋白质产量并没有增加。免疫荧光定位显示牙周韧带细胞产生一种骨相关蛋白--骨结素。此外，在体外这些细胞中还检测到了骨连接蛋白和骨蛋白多糖I(Biglycan)的mRNA水平。这些信息应该有助于阐明这些细胞在牙周组织的调节中所起的作用。
Periodontal ligament cells may have a role in the regulation of hard and soft periodontal tissues, but their specific function has yet to be determined. To evaluate further their role in periodontal homeostasis, they were examined for osteoblast-like behaviour; in vitro no characteristic osteoblastic responsiveness was found. Periodontal ligament cells gave a PGE2- and isoproterenol-mediated cAMP response, but did not respond in a similar fashion to calcitonin or PTH. When exposed to PGE2, isoproterenol, or 1,25(OH)2 vitamin D3, they did not exhibit an increase in protein production, as measured by [35S]-methionine incorporation. Immunofluorescent localization indicated that periodontal ligament cells produced a bone-associated protein, osteonectin. In addition, mRNA levels for osteonectin and bone proteoglycan I (biglycan) were detected in these cells, in vitro. This information should help to clarify the role such cells play in the regulation of periodontal tissues.