Cytoskeleton of the mouse egg and embryo: reorganization of planar elements.
Cytoskeleton of the mouse egg and embryo: reorganization of planar elements.
复制标题
小鼠卵和胚胎的细胞骨架：平面元素的重组。
DOI：
10.1002/cm.970180209
复制
发表时间：
1991
影响因子：
--
通讯作者：
Capco,DG
中科院分区：
文献类型：
--
作者：
Gallicano,GI;McGaughey,RW;Capco,DG
对洗涤剂提取的小鼠卵子和胚胎的检查显示存在两个细胞骨架网络。一种是体细胞中典型的细丝网络，另一种是由大型平面元件组成的网络。后一种细胞骨架结构的个体宽度为60.0±6.8 nm，以发育阶段特异性的方式改变其空间组织。平面元件由直径为10nm的细丝并排排列而成，这些细丝具有20.0±1.6 nm的线性周期性。从不同的发育阶段制备了含有平面元素成分的生化馏分，该馏分中突出的多肽的消失与平面元素的空间组织改变有关。小鼠卵和胚胎的细胞骨架平面元件的超微结构和生物化学与叙利亚仓鼠卵和胚胎的细胞骨架片相当，表明这些细胞骨架成分可能在哺乳动物胚胎发生中具有功能作用。由于这种结构尚未在哺乳动物以外的物种的卵子或胚胎中发现，因此它们的功能可能是哺乳动物胚胎发生所独有的。
Examination of detergent‐extracted mouse eggs and embryos reveals the existence of two cytoskeletal networks. One network is the typical thin filament network observed in somatic cells while the other is composed of large planar elements. These latter cytoskeletal structures, with individual widths of 60.0±6.8 nm, alter their spatial organization in a developmental stage‐specific manner. The planar elements are composed of filaments with a diameter of 10 nm aligned side‐by‐side with these filaments exhibiting a linear periodicity of 20.0±1.6 nm. A biochemical fraction containing components of the planar elements has been prepared from different stages of development and disappearance of prominent polypep‐tides from this fraction correlates with the altered spatial organization of the planar elements. Ultrastructure and biochemistry of cytoskeletal planar elements in eggs and embryos of the mouse are comparable with cytoskeletal sheets of Syrian hamster eggs and embryos, suggesting these cytoskeletal components may have a functional role in mammalian embryogenesis. Because such structures have not been identified in eggs or embryos of species other than mammals, their function may be unique to mammalian embryogenesis.