Investigation of the active biofilm communities on polypropylene filter media in a fixed biofilm reactor for wastewater treatment
Investigation of the active biofilm communities on polypropylene filter media in a fixed biofilm reactor for wastewater treatment
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DOI：
10.1002/jctb.5686
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发表时间：
2018-06
影响因子：
3.4
通讯作者：
I. Naz;Douglas M. Hodgson;Ann Smith;J. Marchesi;S. Sehar;Safia Ahmed;J. Lynch;C. Avignone-Rossa;D. Saroj
中科院分区：
文献类型：
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作者：
I. Naz;Douglas M. Hodgson;Ann Smith;J. Marchesi;S. Sehar;Safia Ahmed;J. Lynch;C. Avignone-Rossa;D. Saroj
研究了温度对好氧固定生物膜反应器（FBR）中聚丙烯（PP）滤料活性生物膜生长的影响。使用高通量测序来探索在10、20和30°C下生长的14天（起始阶段）生物膜的微生物群落的组成和多样性。变形菌门、拟杆菌门和厚壁菌门的成员在从PP-FBR中检索的所有生物膜样品中占主导地位。共鉴定出108个属的细菌，其中一些存在于所有三个反应器中，包括毛球菌属、动胶菌属、气单胞菌属、噬酸菌属和Malikias属等。除了这些共有的种群之外，在10、20和30°C下，个别生物膜样品中还大量发现了某些属，如Brevundimonas（17.1%）、Chitinimonas（10.3%）和Roseateles（39.3%）。通过评估运行期间进水和出水中不同变量（BOD、DO和pH）的变化，估计了PP-FBR中活性微生物群落的代谢能力。与20°C（28.3%）和10°C（28.8%）相比，在30°C下运行的PP-FBR显示出值得注意的BOD去除率（66.6%），这与废水中记录的DO水平一致，在30°C下最高（70.5%），并且随着温度的下降而下降。在30°C下在反应器中观察到显著的废水处理效率，这归因于微生物生物膜的较高相对丰度和多样性。结论在所有主要温度下PP中生理活性生物膜的发展强烈表明该材料适用于在不同操作温度下用于废水处理的FBR中。
BACKGROUND This research is focused on the effect of temperature on the growth of active biofilms on polypropylene (PP) filter media in aerobic fixed biofilm reactors (FBR) for wastewater treatment. RESULTS High‐throughput sequencing was used to explore the composition and diversity of the microbial community of 14‐days‐old (starting phase) biofilms grown at 10, 20 and 30°C. Members of the classes Proteobacteria, Bacteroidetes, and Firmicutes were predominant in all the biofilm samples retrieved from PP‐FBRs. A total of 108 genera of bacteria were identified, with some of them present in all three reactors, including Trichococcus, Zoogloea, Aeromonas, Acidovorax, and Malikias, among others. Besides these shared populations, certain genera were abundantly found in individual biofilm samples, like Brevundimonas (17.1%), Chitinimonas (10.3%) and Roseateles (39.3%), at 10, 20, and 30°C, respectively. The metabolic capabilities of active microbial communities in PP‐FBRs were estimated by assessing the changes in different variables (BOD, DO, and pH) in the influent and effluent during operation. A noteworthy BOD removal (66.6%) was shown by PP‐FBRs operating at 30°C, compared with 20°C (28.3%) and 10°C (28.8%),consistent with the DO levels recorded in the effluents, highest at 30°C (70.5%), and decreasing with declining temperatures. Substantial wastewater treatment efficiencies were observed in the reactors at 30°C, attributable to the higher relative abundance and diversity of microbial biofilms. CONCLUSIONS The development of physiologically active biofilms in PP at all prevailing temperatures strongly suggests that the material is suitable to be employed in FBRs for wastewater treatment at different operational temperatures.