Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1
Purification, crystallization and preliminary X-ray crystallographic analysis of a rice Rac/Rop GTPase, OsRac1
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DOI：
10.1107/s2053230x13033645
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发表时间：
2014-01-01
影响因子：
0.9
通讯作者：
Kojima, Chojiro
中科院分区：
文献类型：
--
作者：
Kosami, Ken-ichi;Ohki, Izuru;Kojima, Chojiro
小GTP酶调节多种关键细胞过程。植物小Rac/Rop GTP酶近来受到广泛关注，因为越来越清楚这些酶调节各种植物细胞过程。水稻Rac/Rop蛋白OsRac 1是活性氧（ROS）产生的关键调节因子，可诱导免疫应答。虽然已经报道了四种植物小GTP酶的结构，但所有这些都是无活性的形式。在这里，OsRac 1被纯化并与GTP类似物50-鸟苷酰亚胺二磷酸（GMPPNP）共结晶。该晶体属于空间群P2（1）2（1）2（1），并收集了1.9埃分辨率的完整数据集。
Small GTPases regulate a large variety of key cellular processes. Plant small Rac/Rop GTPases have recently received broad attention as it is becoming clear that these enzymes regulate various plant cellular processes. OsRac1, a rice Rac/Rop protein, is a key regulator of reactive oxygen species (ROS) production and induces immune responses. Although four structures of plant small GTPases have been reported, all of these were of the inactive form. Here, OsRac1 was purified and co-crystallized with the GTP analogue 50-guanylyl imidodiphosphate (GMPPNP). The crystal belonged to space group P2(1)2(1)2(1) and a complete data set was collected to 1.9 angstrom resolution.