Hypothalamic melanin-concentrating hormone is induced by cold exposure and participates in the control of energy expenditure in rats
Hypothalamic melanin-concentrating hormone is induced by cold exposure and participates in the control of energy expenditure in rats
复制标题
DOI：
10.1210/en.2003-0243
复制
发表时间：
2003-11-01
期刊：
影响因子：
4.8
通讯作者：
Velloso, L
中科院分区：
文献类型：
--
作者：
Pereira-da-Silva, M;Torsoni, M;Velloso, L
恒温动物的短期冷暴露导致较高的产热和食物消耗，伴随着体重减轻。cDNA宏阵列的分析，以确定候选的mRNA物种编码的蛋白质参与产热适应冷。一个cDNA宏阵列分析，证实了RT-PCR，免疫印迹，和RIA，揭示了下丘脑黑色素浓集激素（MCH）的表达增强暴露在寒冷的环境中。用MCH反义寡核苷酸阻断冷暴露大鼠下丘脑MCH的表达，对冷暴露大鼠的摄食行为和体温没有影响。然而，MCH阻断导致体重显著下降，并伴有肝糖原减少、相对体脂增加、绝对和相对肩胛间棕色脂肪组织质量增加、棕色脂肪组织中解偶联蛋白1表达增加以及瘦体重消耗增加。因此，增加下丘脑MCH表达暴露于寒冷的大鼠可能参与的过程中，允许有效地利用能源的产热过程中的产热适应冷。
Short-term cold exposure of homeothermic animals leads to higher thermogenesis and food consumption accompanied by weight loss. An analysis of cDNA-macroarray was employed to identify candidate mRNA species that encode proteins involved in thermogenic adaptation to cold. A cDNA-macroarray analysis, confirmed by RT-PCR, immunoblot, and RIA, revealed that the hypothalamic expression of melanin-concentrating hormone (MCH) is enhanced by exposure of rats to cold environment. The blockade of hypothalamic MCH expression by antisense MCH oligonucleotide in cold-exposed rats promoted no changes in feeding behavior and body temperature. However, MCH blockade led to a significant drop in body weight, which was accompanied by decreased liver glycogen, increased relative body fat, increased absolute and relative interscapular brown adipose tissue mass, increased uncoupling protein 1 expression in brown adipose tissue, and increased consumption of lean body mass. Thus, increased hypothalamic MCH expression in rats exposed to cold may participate in the process that allows for efficient use of energy for heat production during thermogenic adaptation to cold.