An invertebrate model to evaluate virulence in Aspergillus fumigatus: The role of azole resistance
An invertebrate model to evaluate virulence in Aspergillus fumigatus: The role of azole resistance
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DOI：
10.1093/mmy/myt022
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发表时间：
2014-04-01
期刊：
影响因子：
2.9
通讯作者：
Cuenca-Estrella, M.
中科院分区：
文献类型：
--
作者：
Gomez-Lopez, A.;Forastiero, A.;Cuenca-Estrella, M.
烟曲霉麦角固醇生物合成途径中的不同突变对发病机制的影响已经使用一个简单的无脊椎动物的微型主机，大蜡螟毛虫进行了评估。研究了一组菌株，包括临床分离株和在cyp51A基因突变的同基因突变体，赋予唑类耐药。所有菌株均表现出相似的体外生长模式，并且对昆虫幼虫具有相同的毒性。这些结果表明，在A.烟曲霉获得这种特殊的唑类抗性机制并不意味着毒力的任何显著变化。G. Mellonella可能为A.烟曲霉，有助于产生一个假设，可以在哺乳动物模型的精细实验中进一步测试。
The impact of different mutations in the Aspergillus fumigatus ergosterol biosynthesis pathway on pathogenesis has been evaluated using a simple invertebrate mini host, the caterpillar Galleria mellonella. A set of strains that includes clinical isolates and isogenic mutants with mutations at the cyp51A gene conferring azole resistance were studied. All strains demonstrated a similar in vitro growth pattern and are equally virulent against the insect larvae. These results suggest that in A. fumigatus acquisition of this particular azole-resistance mechanism would not imply any significant change in virulence. G. mellonella may provide a convenient and inexpensive model for the in vivo prescreening of mutants of A. fumigatus, contributing to the generation of a hypotheses that can be further tested in refined experiments in mammalian models.