A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation
A Method for Microalgae Proteomics Analysis Based on Modified Filter-Aided Sample Preparation
复制标题
基于改进过滤辅助样品制备的微藻蛋白质组学分析方法
DOI：
10.1007/s12010-017-2473-9
复制
发表时间：
2017-11-01
影响因子：
3
通讯作者：
Tian, Jing
中科院分区：
文献类型：
--
作者：
Li, Song;Cao, Xupeng;Tian, Jing
随着微藻生物燃料研究的快速发展，微藻蛋白质组学研究也迅速增加。自2009年以来，过滤器辅助样品制备（FASP）方法是广泛使用的蛋白质组学样品制备方法。针对微藻细胞的特点，提出了一种基于改进的过滤辅助样品制备（mFASP）的微藻蛋白质组学分析方法。以莱氏衣原体为模型，采用标准LC-MS/MS对制备的样品进行检测，并与文献报道的结果进行比较。结果表明，mFASP适用于微藻蛋白质组学研究的大多数场合。
With the fast development of microalgal biofuel researches, the proteomics studies of microalgae increased quickly. A filter-aided sample preparation (FASP) method is widely used proteomics sample preparation method since 2009. Here, a method of microalgae proteomics analysis based on modified filter-aided sample preparation (mFASP) was described to meet the characteristics of microalgae cells and eliminate the error caused by over-alkylation. UsingChlamydomonas reinhardtiias the model, the prepared sample was tested by standard LC-MS/MS and compared with the previous reports. The results showed mFASP is suitable for most of occasions of microalgae proteomics studies.