Endogenous retroviral envelope antigen in plasma cells.
Endogenous retroviral envelope antigen in plasma cells.
复制标题
浆细胞中的内源性逆转录病毒包膜抗原。
作者：
M. Halpern;D. Ewert;L. Flores;K. Lin;J. England
先前观察到SRBC致敏的15 I5 × 71鸡脾IG（+）B细胞表达内源性逆转录病毒包膜抗原，提示该抗原的表达可能是15 I5及相关毒株浆细胞的一般特性。为了研究这种可能性，使用哈德腺中存在的浆细胞进行抗病毒血清的免疫荧光测定。内源性逆转录病毒包膜抗原可在15I5 X 71、15I5 X 72和15I5系鸡的浆细胞中检测到，但在72、15B或63系鸡的浆细胞中检测不到。与15I5 X 71囊细胞相比，15I5 X 71浆细胞中可检测到更高水平的逆转录病毒包膜抗原。在15I5浆细胞中存在逆转录病毒包膜抗原，加上在这些细胞中没有可检测水平的逆转录病毒p27相关抗原，表明前者的表达受ev 6基因座的控制。
Previous observations that endogenous retroviral envelope antigen is expressed in splenic Ig(+) B cells of SRBC-sensitized 15I5 X 71 chickens suggested that expression of this antigen might be a general property of plasma cells in 15I5 and related strains. To investigate this possibility, immunofluorescence assays with antiviral sera were carried out using plasma cells present in Harder glands. Endogenous retroviral envelope antigen was detectable in plasma cells of line 15I5 X 71, 15I5 X 72, and 15I5 chickens but not in plasma cells of line 72, 15B, or 63 chickens. Higher levels of retroviral envelope antigen were detectable in 15I5 X 71 plasma cells as compared to 15I5 X 71 bursal cells. The presence of retroviral envelope antigen in 15I5 plasma cells, coupled with the absence of detectable levels of retroviral p27-related antigen in these cells, suggested that expression of the former is governed by the ev 6 genetic locus.