Gene expression profiling of porokeratosis
Gene expression profiling of porokeratosis
复制标题
汗孔角化症的基因表达谱
DOI：
10.1111/j.1600-0560.2008.01026.x
复制
发表时间：
2008-11-01
影响因子：
1.7
通讯作者：
Zheng, Zhi-Zhong
中科院分区：
文献类型：
--
作者：
Zhang, Zheng-Hua;Wang, Zhi-Min;Zheng, Zhi-Zhong
背景：汗孔角化症(PK)是一组异质性角化性疾病，临床表现多样。PK可能在临床和分子水平上与银屑病表现出相似之处。方法：我们利用Illumina(R)BeadArray(TM)平台研究了不同亚型PK患者皮损和非皮损组织的转录水平。结果：共鉴定出37个上调基因，包括创伤诱导角蛋白、参与表皮分化的S100钙结合蛋白基因以及参与细胞间通讯和免疫反应的基因。据我们所知，这是第一次研究PK病变的免疫学特征。结论：我们报道了含有PK病变的角质形成细胞(KCs)激活并过度表达了创伤诱导的角蛋白基因，该基因似乎与其他参与介导表皮分化、细胞间通讯和免疫的基因协同调节。这项研究从基因图谱的角度，支持PK中的基因错误调控与银屑病相似。我们的数据表明，参与T细胞介导的免疫反应途径和KCs激活的基因在PK的发病机制中起着关键作用。
Background: Porokeratosis (PK) represents a heterogeneous group of disorders of keratinization and has a wide variety of clinical manifestations. PK may exhibit similarities with psoriasis at both clinical and molecular levels. The genetic basis and pathogenesis for PK remain elusive.Methods: We studied the transcriptional profiles of three pairwise lesional and uninvolved skin biopsies from patients with different subtypes of PK using the Illumina((R)) BeadArray (TM) platform.Results: A total of 37 upregulated genes were identified in our study, including wound-induced keratins, S100 calcium-binding protein genes involved in epidermal differentiation, as well as genes involved in mediating intercellular communication and the immune response. To our knowledge, this is the first study that characterizes the immune profile of PK lesions.Conclusions: Here, we report that keratinocytes (KCs)-harboring lesions have activated and overexpressed wound-induced keratin genes, which appear to be coregulated with other genes involved in mediating epidermal differentiation, intercellular communication and immunity. This study, from the perspective of gene profiling, supports that gene misregulation in PK mimics that of psoriasis. Our data indicate that the genes implicated in the T-cell-mediated immune response pathway and activation of KCs play a key role in the pathogenesis of PK.