Curcumin inhibits the proliferation and mineralization of cultured osteoblasts
Curcumin inhibits the proliferation and mineralization of cultured osteoblasts
复制标题
DOI：
10.1016/j.ejphar.2006.01.028
复制
发表时间：
2006-03-18
影响因子：
5
通讯作者：
Hagiwara, H
中科院分区：
文献类型：
--
作者：
Notoya, M;Nishimura, H;Hagiwara, H
在培养的大鼠颅骨成骨细胞(ROB细胞)上观察了姜黄素对成骨细胞代谢的影响。5×10~(-6)~1~10~(-5)M的姜黄素可明显抑制ROB细胞的增殖，1×10~(-5)M的Curcunifti不能诱导ROB细胞的凋亡，但使细胞停滞于细胞周期的G1期。此外，姜黄素还可刺激p21(WAF1/CIP1)的mRNA表达，从而抑制细胞周期蛋白依赖性蛋白激酶的活性，并抑制历史H1的磷酸化。此外，Curcurnin还降低了钙沉积和矿化结节的形成速度。提示姜黄素可能通过p21(WAF1/CIP1)的表达抑制成骨细胞的增殖和矿化。(C)2006年，爱思唯尔出版。
The effects of curcumin, which is an important constituent of rhizomes of the plant Curcuma longa Linn, on the metabolism of osteoblasts were examined in cultures of rat calvarial osteoblastic cells (ROB cells). The proliferation of cells was markedly inhibited upon exposure of cells to curcumin at 5 x 10(-6) to 1-10(-5) M. Curcunifti at 1 X 10(-5) M did not induce apoptosis in ROB cells but arrested cells at the G1 phase of the cell cycle. In addition, curcumin stimulated the expression of mRNA for p21(WAF1/CIP1) which inhibits the activity of cyclin-dependent kinases, and inhibited the phosphorylation of historic H1. Furthermore, curcurnin reduced the rate of deposition of calcium and the formation of mineralized nodules. Our results indicate that curcumin might inhibit the proliferation and mineralization of osteoblastic cells through the expression of p21(WAF1/CIP1). (c) 2006 Published by Elsevier B.V.