Deterministic separation of cancer cells from blood at 10 mL/min
Deterministic separation of cancer cells from blood at 10 mL/min
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DOI：
10.1063/1.4758131
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发表时间：
2012-12-01
期刊：
影响因子：
1.6
通讯作者：
Sturm, James C.
中科院分区：
文献类型：
--
作者：
Loutherback, Kevin;D'Silva, Joseph;Sturm, James C.
循环肿瘤细胞（CTC）和循环癌症簇和基质细胞已经在恶性癌症患者的血液中被鉴定，并且可以用作疾病严重程度的诊断，评估不同治疗策略的功效，并可能确定转移性侵袭的最终位置以进行可能的治疗。因此，迫切需要分离、增殖和表征活的CTC和癌细胞簇及其相关的基质细胞。在这里，我们提出了一种用于mL/min流速的微流体装置，使用确定性横向位移（DLD）阵列从血液中连续捕获活的CTC。我们在此表明，DLD阵列装置可以从血液中分离CTC，在高达10 mL/min的体积流速下捕获效率大于85% CTC，而对细胞活力没有影响。版权所有2012作者。本文是在知识共享署名3.0未移植许可证下发布的。[http：//dx.doi.org/10.1063/1.4758131]
Circulating tumor cells (CTCs) and circulating clusters of cancer and stromal cells have been identified in the blood of patients with malignant cancer and can be used as a diagnostic for disease severity, assess the efficacy of different treatment strategies and possibly determine the eventual location of metastatic invasions for possible treatment. There is thus a critical need to isolate, propagate and characterize viable CTCs and clusters of cancer cells with their associated stroma cells. Here, we present a microfluidic device for mL/min flow rate, continuous-flow capture of viable CTCs from blood using deterministic lateral displacement (DLD) arrays. We show here that a DLD array device can isolate CTCs from blood with capture efficiency greater than 85% CTCs at volumetric flow rates of up to 10 mL/min with no effect on cell viability. Copyright 2012 Author(s). This article is distributed under a Creative Commons Attribution 3.0 Unported License. [http://dx.doi.org/10.1063/1.4758131]