ANXIOGENIC EFFECTS OF A LACTOBACILLUS, INULIN AND THE SYNBIOTIC ON HEALTHY JUVENILE RATS
ANXIOGENIC EFFECTS OF A LACTOBACILLUS, INULIN AND THE SYNBIOTIC ON HEALTHY JUVENILE RATS
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DOI：
10.1016/j.neuroscience.2017.06.064
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发表时间：
2017-09-17
期刊：
影响因子：
3.3
通讯作者：
Bravo, Javier A.
中科院分区：
文献类型：
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作者：
Barrera-Bugueno, Camila;Realini, Ornella;Bravo, Javier A.
肠道菌群干预，包括益生菌和益生元的使用可以改变成年动物和健康志愿者的行为。然而，人们对它们对年轻人的影响知之甚少。为了研究这一点，雄性Sprague Dawley大鼠（出生后第21天，PND21）通过饮用水摄入干酪乳杆菌54-2-33 (10(4)cfu/ml)，菊粉作为益生元（16 mg/ml），或两者同时（合成）饮用14天。对照大鼠只喝水。在PND34和35时分别评价开阔场（OF）和高架迷宫（EPM）行为。EPM后30min，取脑和干血，测定海马5-HT1A （mRNA和蛋白）和血浆皮质酮（CORT）水平。与对照组相比，乳杆菌、菊粉和合成菌治疗的大鼠进入OF中心的次数更少，在其外围停留的时间更长。合成菌喂养的大鼠比益生菌和菊粉喂养的大鼠探索EPM张开的手臂的时间更长。合成菌喂养的大鼠，而不是乳酸菌喂养的大鼠和菊粉喂养的大鼠，epm诱发的CORT水平低于对照组。在初始队列中评估的基础CORT水平，乳杆菌和菊粉喂养的大鼠高于对照组。合成饲大鼠牙状回和氨角1层（CA1） 5-HT1A mRNA水平高于其他各组，而细菌饲大鼠海马5-HT1A蛋白水平低于对照组。5-HT1A mRNA的变化提示肠道微生物对海马基因表达机制的复杂影响，可能涉及内源性/外源性细菌和益生元的相互作用。重要的是，年龄也可能影响他们的行为结果。综上所述，这些数据表明，对幼鼠微生物群的干预引起了应激下早期行为的改变，显然是以下丘脑-垂体-肾上腺轴独立的方式发生的。(c) 2017年。Elsevier Ltd.出版。版权所有。
Gut microbiota interventions, including probiotic and prebiotic use can alter behavior in adult animals and healthy volunteers. However, little is known about their effects in younger individuals. To investigate this, male Sprague Dawley rats (post-natal day 21, PND21) received Lactobacillus casei 54-2-33 (10(4) cfu/ml), inulin as prebiotic (16 mg/ml), or both together (synbiotic) via drinking water for 14 days. Control rats received water alone. Open field (OF) and elevated plus maze (EPM) behaviors were evaluated at PND34 and 35, respectively. 30 min after EPM, brains and trunk blood were collected to evaluate hippocampal 5-HT1A (mRNA and protein) and plasma corticosterone (CORT). Lactobacillus, inulin and synbiotic-treated rats had fewer entries to the OF's center and spent more time in its periphery than controls. Synbiotic-fed rats explored the EPM's open arms longer than probiotic and inulin-fed rats. Synbiotic, but not Lactobacillus nor inulin-fed rats had lower levels of EPM-evoked CORT than controls. Basal CORT levels, evaluated in a naive cohort, were higher in Lactobacillus-and inulin-fed rats than controls. In naive synbiotic-fed rats, 5-HT1A mRNA levels were higher in dentate gyrus and cornus ammonis 1 layer (CA1), than in all other naive groups, while hippocampal 5-HT1A protein levels were lower in bacteria fed rats than controls. 5-HT1A mRNA changes suggest complex effects of gut microbes on hippocampal gene expression machinery, probably involving endogenous/exogenous bacteria and prebiotics interactions. Importantly, age might also influence their behavioral outcomes. Together, these data suggest that interventions in young rat microbiota evoke early behavioral changes upon stress, apparently in a hypothalamus-pituitary-adre nal axis independent fashion. (C) 2017 IBRO. Published by Elsevier Ltd. All rights reserved.