ISOLATION OF COMPONENTS OF THE CHLOROPLAST PROTEIN IMPORT MACHINERY
ISOLATION OF COMPONENTS OF THE CHLOROPLAST PROTEIN IMPORT MACHINERY
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DOI：
10.1126/science.7973649
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发表时间：
1994-11-11
期刊：
影响因子：
56.9
通讯作者：
BLOBEL, G
中科院分区：
文献类型：
--
作者：
SCHNELL, DJ;KESSLER, F;BLOBEL, G
叶绿体蛋白质进口机制的组成部分是通过一种程序分离的，在这种程序中，进口机制与标记的进口底物一起在体外进行，在产生主要是叶绿体包膜结合的进口中间体的条件下进行。随后对包膜的洗涤剂增溶表明，6个包膜多肽与进口中间体进行了特异性和明显的化学计量化共纯化。其中四种多肽是外膜进口机制的组成部分，与早期进口中间体有关。其中两种多肽已被表征。一个是热休克蛋白hsp70的同源物；另一种是通道蛋白候选者。
Components of the protein import machinery of the chloroplast were isolated by a procedure in which the import machinery was engaged in vitro with a tagged import substrate under conditions that yielded largely chloroplast envelope-bound import intermediates. Subsequent detergent solubilization of envelope membranes showed that six envelope polypeptides copurified specifically and, apparently, stoichiometrically with the import intermediates. Four of these polypeptides are components of the outer membrane import machinery and are associated with early import intermediates. Two of these polypeptides have been characterized. One is a homolog of the heat shock protein hsp70; the other one is a channel-protein candidate.