Engineered Vascularized Flaps, Composed of Polymeric Soft Tissue and Live Bone, Repair Complex Tibial Defects
Engineered Vascularized Flaps, Composed of Polymeric Soft Tissue and Live Bone, Repair Complex Tibial Defects
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DOI：
10.1002/adfm.202008687
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发表时间：
2021-02-18
影响因子：
19
通讯作者：
Levenberg, Shulamit
中科院分区：
文献类型：
--
作者：
Redenski, Idan;Guo, Shaowei;Levenberg, Shulamit
复杂的大型缺损区的功能性再生需要软组织和硬组织的移植。此外，这些移植物内的骨结构需要广泛的血管供应以供在植入期间存活和代谢。软组织蒂常用于骨性结构的血管化。然而，制作这些移植物所需的大量自体组织采集仍然是一个主要的程序缺陷。在目前的工作中，合成的软组织基质和脱细胞的骨，在体内结合，形成具有自身血管供应的新生复合组织，形成复合组织。使用牙髓干细胞(DPSCs)和人脂肪微血管内皮细胞(HAMECs)对软组织基质进行血管预血管化可以促进脱细胞骨骼中的血管发育。此外，利用脂肪来源的间充质基质细胞构建的骨结构的成骨诱导对新生组织矿化成分中的微血管组织有积极的影响。最终，这些用作轴向重建皮瓣的新组织支持长期的骨缺损修复，以及肌肉缺损的桥接。对于需要移植存活移植物的患者来说，这里描述的复合皮瓣可能有助于避免侵入性的自体组织采集。
Functional regeneration of complex large-scaled defects requires both soft- and hard-tissue grafts. Moreover, bone constructs within these grafts require an extensive vascular supply for survival and metabolism during the engraftment. Soft-tissue pedicles are often used to vascularize bony constructs. However, extensive autologous tissue-harvest required for the fabrication of these grafts remains a major procedural drawback. In the current work, a composite flap is fabricated using synthetic soft-tissue matrices and decellularized bone, combined in vivo to form de novo composite tissue with its own vascular supply. Pre-vascularization of the soft-tissue matrix using dental pulp stem cells (DPSCs) and human adipose microvascular endothelial cells (HAMECs) enhances vascular development within decellularized bones. In addition, osteogenic induction of bone constructs engineered using adipose derived mesenchymal stromal cells positively affects micro-capillary organization within the mineralized component of the neo-tissue. Eventually, these neo-tissues used as axial reconstructive flaps support long-term bone defect repair, as well as muscle defect bridging. The composite flaps described here may help eliminate invasive autologous tissue-harvest for patients in need of viable grafts for transplantation.