Generation and characterization of a Notch1 signaling-specific reporter mouse line
Generation and characterization of a Notch1 signaling-specific reporter mouse line
复制标题
DOI：
10.1002/dvg.22030
复制
发表时间：
2012-09-01
期刊：
影响因子：
1.5
通讯作者：
Radtke, Freddy
中科院分区：
文献类型：
--
作者：
Smith, Emma;Claudinot, Stephanie;Radtke, Freddy
通过Notch1受体的信号对于控制胚胎生命中的许多发育过程以及成人组织的动态平衡和疾病是必不可少的。由于Notch1信号转导的结果高度依赖于上下文，其在许多器官中的确切生理和病理作用尚不清楚，因此在体内定位和识别接收活跃Notch1信号的细胞是非常有意义的。在这里，我们报告了一个BAC转基因小鼠品系N1-Gal4VP16的产生和特征，当它与Gal4反应的报告小鼠品系杂交时，可以在体内鉴定正在经历活跃Notch1信号的细胞。对胚胎和成年N1-Gal4VP16小鼠的分析表明，转基因的激活模式与先前观察到的内源性Notch1受体的激活模式一致。因此，这个新颖的报告鼠系提供了一个独特的工具来专门研究体内Notch1信号的空间和时间方面。创世纪50：700710,2012年。(C)2012年威利期刊公司。
Signaling through the Notch1 receptor is essential for the control of numerous developmental processes during embryonic life as well as in adult tissue homeostasis and disease. Since the outcome of Notch1 signaling is highly context-dependent, and its precise physiological and pathological role in many organs is unclear, it is of great interest to localize and identify the cells that receive active Notch1 signals in vivo. Here, we report the generation and characterization of a BAC-transgenic mouse line, N1-Gal4VP16, that when crossed to a Gal4-responsive reporter mouse line allowed the identification of cells undergoing active Notch1 signaling in vivo. Analysis of embryonic and adult N1-Gal4VP16 mice demonstrated that the activation pattern of the transgene coincides with previously observed activation patterns of the endogenous Notch1 receptor. Thus, this novel reporter mouse line provides a unique tool to specifically investigate the spatial and temporal aspects of Notch1 signaling in vivo. genesis 50:700710, 2012. (c) 2012 Wiley Periodicals, Inc.