Effects of topical elk velvet antler on cutaneous wound healing in streptozotocin-induced diabetic rats.
Effects of topical elk velvet antler on cutaneous wound healing in streptozotocin-induced diabetic rats.
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DOI：
10.1089/acm.2004.10.835
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发表时间：
2004-11
影响因子：
2.6
通讯作者：
J. Mikler;C. Theoret;Jerry C High
中科院分区：
文献类型：
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作者：
J. Mikler;C. Theoret;Jerry C High
目的创伤修复是一个精心安排的过程，涉及细胞、分子、生理和生化相互作用，以恢复受损组织的完整性。鹿角的循环更换需要快速的再生生长，在许多方面类似于组织修复过程中遇到的情况。调控这些过程的分子机制尚不完全清楚，但越来越明显的是，生长因子是重要的介体。以往的研究表明，麋鹿鹿茸(EVA)含有多种生长因子，水溶性提取物在体外可以刺激真皮成纤维细胞生长。设计采用全层皮肤创面模型评价EVA水溶性提取物对链脲佐菌素诱导的糖尿病大鼠创面愈合的影响。动物被随机选择接受对照组或EVA凝胶的局部应用。伤口的每日照片用于测量伤口闭合率。术后第2天和第10天获得创缘活检组织学评估，并用酶联免疫吸附试验测量转化生长因子-β1(1)浓度。结果术后第6天，EVA局部凝胶治疗组创面面积明显小于对照组，创面组织中的转化生长因子-β(1)蛋白表达与对照组相比无明显差异。结论EVA水溶性提取物外用可加速糖尿病大鼠皮肤创面的修复。有必要进行进一步的研究，以揭示EVA促进伤口闭合的机制，并确定该化合物是否为治疗正常和受损伤口的一种经济的药理药物。
OBJECTIVE Wound repair is a finely orchestrated process involving cellular, molecular, physiologic, and biochemical interactions that restore the integrity of damaged tissue. Cyclic replacement of deer antlers requires rapid regenerative growth, in many ways analogous to that encountered during tissue repair. Molecular mechanisms regulating these processes are not fully understood, but it is increasingly apparent that growth factors are important mediators. Previous studies have shown that elk velvet antler (EVA) contains various growth factors and that a water-soluble extract stimulates dermal fibroblast growth in vitro. DESIGN The efficacy of EVA water-soluble extract on wound healing in streptozotocin-induced diabetic rats was EVAluated using a full-thickness cutaneous wound model. Animals were randomly selected to receive topical application of either control or EVA gel. Daily photographs of the wounds served to measure the rate of wound closure. Wound-edge biopsies obtained on postoperative days 2 and 10 allowed histologic evaluation and measurement of transforming growth factor-beta 1 (TGF-beta (1)) concentrations by enzyme-linked immunoabsorbent assay. RESULTS Wounds treated with the EVA topical gel were significantly smaller by postoperative day 6. TGF- beta (1) protein expression was not different in EVA-treated wounds compared to control wounds. CONCLUSIONS This study indicates that topical treatment with an EVA water-soluble extract accelerates repair of cutaneous wounds in diabetic rats. Further studies are warranted to reveal the mechanisms involved in EVA enhancement of wound closure and to determine if this compound is an economical pharmacologic agent in the treatment of normal and compromised wounds.