The fraction of cortical GABAergic neurons is constant from near the start of cortical neurogenesis to adulthood.
The fraction of cortical GABAergic neurons is constant from near the start of cortical neurogenesis to adulthood.
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DOI：
10.1523/jneurosci.6412-11.2012
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发表时间：
2012-04-04
期刊：
影响因子：
--
通讯作者：
Stevens CF
中科院分区：
文献类型：
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作者：
Sahara S;Yanagawa Y;O'Leary DD;Stevens CF
在许多新皮质区域和物种中，大约五分之一的神经元具有 GABA 能，在成人回路中形成抑制和兴奋之间的关键平衡。在发育过程中，皮层 GABA 能神经元在腹侧端脑中产生，并迁移到发育中的皮层，在那里产生兴奋性谷氨酸能神经元。我们在这里问：在发育过程中，成人 GABA 能/谷氨酸能神经元的比例是何时首次建立的？为了回答这个问题，我们确定了从胚胎第 10.5 天 (E10.5) 到出生后第 28 天 (P28) 的小鼠中所有新皮质 GABA 能神经元将变得抑制性 (GAD67+) 的比例。我们发现，这个分数接近成人值的 1/5，从皮质生成早期（E14.5，此时 GAD67+ 神经元仍然切向迁移到皮质）开始，并在大脑发育的其余部分继续保持相同的 1/5 值。因此，我们的数据表明，五分之一的 GABA 能神经元在神经元迁移过程中以及显着的突触形成之前就已经建立了。
About one in five neurons is GABAergic in many neocortical areas and species, forming a critical balance between inhibition and excitation in adult circuits. During development, cortical GABAergic neurons are generated in ventral telencephalon and migrate up to developing cortex where the excitatory glutamatergic neurons are born. We ask here: when during development is the adult GABAergic/glutamatergic neuron ratio first established? To answer this question, we have determined the fraction of all neocortical GABAergic neurons that will become inhibitory (GAD67+) in mice from embryonic day 10.5 (E10.5) to postnatal day 28 (P28). We find that this fraction is close to 1/5, the adult value, starting from early in corticogenesis (E14.5, when GAD67+ neurons are still migrating tangentially to the cortex) and continuing at the same 1/5 value throughout the remainder of brain development. Thus our data indicate the one-in-five fraction of GABAergic neurons is already established during their neuronal migration and well before significant synapse formation.