Response to: ‘Anti-Ku antibodies: important points to consider’ by Mahler et al
Response to: ‘Anti-Ku antibodies: important points to consider’ by Mahler et al
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回应：Mahler 等人的“抗 Ku 抗体：需要考虑的要点”
DOI：
10.1136/annrheumdis-2019-216595
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发表时间：
2019
影响因子：
27.4
通讯作者：
Akiyama Masashi
中科院分区：
文献类型：
--
作者：
Ogawa-Momohara Mariko;Muro Yoshinao;Akiyama Masashi
我们感谢作者 1 对我们的论文 2 的兴趣，并感谢有机会回应所提出的观点。我们同意 Mahler 等人的观点，即“SLE 经常表达多种自身抗体，并且血清伴有混合 HEp-2 IIF 模式非常常见”。我们采用与 Spielmann 等人 3 在他们的研究中使用的相同方法进行抗 Ku 筛查，以避免漏掉多个自身抗体阳性病例。 Spielmann 小组纳入了显示多种 ANA 模式（ICAP 命名法：AC-1、AC-4 或 AC-5）的 ANA 阳性血清进行 ELISA 分析。尽管 Mahler 等人指出，“根据我们的经验，抗 Ku 抗体经常在 SLE 患者中发现，可能与肌病的重叠特征相关”，但在其参考研究中的 4 名抗 Ku 抗体阳性肌炎患者中，只有 2 名患者具有抗 Ku 抗体阳性血清。 系统性红斑狼疮（SLE）与肌炎重叠。 3 鉴于研究 4 中的 33 名抗 Ku 阳性 SLE 患者没有肌炎，看来 SLE 和肌炎重叠的可能性较小。我们在讨论抗 Ku 相关临床表型的频率时需要小心。我们使用两种商业 ELISA 试剂盒（MBL，名古屋，日本）检测抗 dsDNA 和抗 ssDNA 抗体。这些试剂盒在日本常用，并且包含在临床实验室测试的健康保险范围内。正如 Mahler 等人指出的那样，抗 dsDNA 测定之间存在差异，1 我们需要使用以 Crithidia luciliae 作为底物的免疫荧光测试进行确认。
We thank the authors 1 for their interest in our paper 2 and are grateful for the opportunity to respond to the points raised. We agree with the opinion of Mahler et al that ‘SLE frequently express many autoantibodies and sera accompanied by mixed HEp-2 IIF pattern were very common’. We adopted the same methods for anti-Ku screening that Spielmann et al 3 used in their study to avoid missing multiple autoantibody-positive cases. The Spielmann group included ANA-positive sera showing several ANA patterns (ICAP nomenclature: AC-1, AC-4 or AC-5) for ELISA analysis.Although Mahler et al noted that ‘In our experience, anti-Ku antibodies are frequently found in SLE patients potentially associated with overlap features of a myopathy’, of the four anti-Ku antibody-positive myositis patients in their referenced study, only two patients had systemic lupus erythematosus (SLE) overlapping with myositis. 3 Given that 33 anti-Ku-positive SLE patients in their study 4 did not have myositis, it seems that SLE and myositis are less likely to overlap. We need to be careful in discussing the frequency of anti-Ku-associated clinical phenotypes. We detected anti-dsDNA and anti-ssDNA antibodies by using two commercial ELISA kits (MBL, Nagoya, Japan). These kits are commonly used in Japan and are covered by health insurance for clinical laboratory tests. As Mahler et al noted that there is variability between anti-dsDNA assays, 1 we need to use immunofluorescence tests with Crithidia luciliae as a substrate for confirmation.
抗 Ku 抗体阳性患者很少同时患有系统性红斑狼疮和肌炎
影响因子：
27.4
作者：
Ogawa-Momohara Mariko;Muro Yoshinao;Akiyama Masashi
通讯作者：
Akiyama Masashi
影响因子：
27.4
作者：
Spielmann, Lionel;Nespola, Benoit;Meyer, Alain
通讯作者：
Meyer, Alain
影响因子：
2.6
作者：
Mahler, M.;Swart, A.;Fritzler, M. J.
通讯作者：
Fritzler, M. J.