The solution structure of the adhesion protein Bd37 from Babesia divergens reveals structural homology with eukaryotic proteins involved in membrane trafficking
The solution structure of the adhesion protein Bd37 from Babesia divergens reveals structural homology with eukaryotic proteins involved in membrane trafficking
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DOI：
10.1016/j.jmb.2007.08.019
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发表时间：
2008-01-11
影响因子：
5.6
通讯作者：
Roumestand, Christian
中科院分区：
文献类型：
--
作者：
Delbecq, Stephane;Auguin, Daniel;Roumestand, Christian
分歧巴贝斯虫是欧洲牛巴贝斯虫病的顶复门病原体：这种感染导致生长和泌乳减少，因此由于这种寄生虫造成的经济损失足以需要开发疫苗。B的主要表面抗原。趋异因子被描述为锚定在裂殖子表面的37 kDa蛋白糖基磷脂酰肌醇（GPI）。已经证明了Bd 37的免疫预防潜力，并且我们在这里使用NMR光谱法呈现了Bd 37（Delta-Bd 37）的27 kDa结构核心的高分辨率溶液结构。使用额外的小角X射线散射（SAXS）数据获得了整个蛋白质的模型。Bd 37的3D结构的知识允许其表面上抗体的精确表位作图。有趣的是，Delta-Bd 37的几何结构揭示了与外囊亚基Exo 84 p C-末端区域的有趣的相似性，外囊亚基Exo 84 p C-末端区域是一种在囊泡运输中具有直接意义的真核蛋白。这有力地表明，顶复门在结构和功能上与真核细胞中用于胞内和胞吐的分子机器相似的平行分子机器中发展。(C)2007爱思唯尔有限公司版权所有。
Babesia divergens is the Apicomplexa agent of the bovine babesiosis in Europe: this infection leads to growth and lactation decrease, so that economical losses due to this parasite are sufficient to require the development of a vaccine. The major surface antigen of B. divergens has been described as a 37 kDa protein glycosyl phosphatidyl inositol (GPI)anchored at the surface of the merozoite. The immuno-prophylactic potential of Bd37 has been demonstrated, and we present here the high-resolution solution structure of the 27 kDa structured core of Bd37 (Delta-Bd37) using NMR spectroscopy. A model for the whole protein has been obtained using additional small angle X-ray scattering (SAXS) data. The knowledge of the 3D structure of Bd37 allowed the precise epitope mapping of antibodies on its surface. Interestingly, the geometry of Delta-Bd37 reveals an intriguing similarity with the exocyst subunit Exo84p C-terminal region, an eukaryotic protein that has a direct implication in vesicle trafficking. This strongly suggests that Apicomplexa have developed in parallel molecular machines similar in structure and function to the ones used for endo- and exocytosis in eukaryotic cells. (C) 2007 Elsevier Ltd. All rights reserved.