Di-5-ANEQ(F)PTEA Offers Better Performance than Di-4-ANEQ(F)PTEA for In-Situ Cardiac Optical Mapping.
Di-5-ANEQ(F)PTEA Offers Better Performance than Di-4-ANEQ(F)PTEA for In-Situ Cardiac Optical Mapping.
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Di-5-ANEQ(F)PTEA 在原位心脏光学测绘方面比 Di-4-ANEQ(F)PTEA 具有更好的性能。
DOI：
10.1109/embc40787.2023.10340445
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发表时间：
2023
期刊：
影响因子：
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通讯作者：
Rogers,JackM
中科院分区：
文献类型：
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作者：
Zhang,Hanyu;Patton,HaleyN;Wood,GarrettA;Yan,Ping;Loew,LeslieM;Acker,CoreyD;Walcott,GregoryP;Rogers,JackM
心脏光学测绘传统上是在离体、运动停止的心脏中进行的。最近，运动校正技术和长波长电压敏感染料（vsd）使得原位心脏光学成像成为可能。然而，已观察到室间隔缺损可迅速从血流灌注的原位心脏中清除。在这项研究中，我们评估了新开发的VSD， di-5-ANEQ(F)PTEA，相对于早期的VSD， di-4-ANEQ(F)PTEA的性能。我们发现di-5-ANEQ(F)PTEA持续时间超过3倍，产生改进的信噪比，并且不会不可接受地延长负载。临床相关性-光学制图为心律失常提供了许多见解，但传统上仅限于离体制剂。目前的研究结果扩展了光学测绘在更现实的体内环境中的效用，并可能最终使其在患者中使用。
Cardiac optical mapping has traditionally been performed in ex-vivo, motion-arrested hearts. Recently, in-situ cardiac optical mapping has been made possible by both motion correction techniques and long-wavelength voltage sensitive dyes (VSDs). However, VSDs have been observed to wash out quickly from blood-perfused in-situ hearts. In this study, we evaluate the performance of a newly developed VSD, di-5-ANEQ(F)PTEA, relative to an earlier VSD, di-4-ANEQ(F)PTEA. We find that di-5-ANEQ(F)PTEA persists over 3 times longer, produces improved signal-to-noise ratio, and does not prolong loading unacceptably.Clinical Relevance—Optical mapping has provided many insights into cardiac arrhythmias, but has traditionally been limited to ex-vivo preparations. The present findings extend the utility of optical mapping in the more realistic in-vivo setting and may eventually enable its use in patients.